BMP-7 蛋白, Human (His)

Bone morphogenetic protein 7 (BMP-7) is a polymorphic ligand protein belonging to the TGF-β family. BMP-7 is involved in regulating the proliferation, invasion and migration of cancer cells and is associated with a variety of human tumors^[1]. BMP-7 binds ALK2 or ACVR2A/BMPR2 excitation signal, which is terminated by SMADs regulation^[2]. BMP-7 is involved in the BMP-7-SMad1/5/9 signaling pathway, which is associated with the epithelial-mesenchymal transition (EMT) process^[1]. BMP-7 also eliminates vascular inflammation, maintains vascular integrity^[3], reduces vascular calcification, and stimulates in situ phosphate ossification deposition^[4]. The total length of human BMP-7 protein is 431 amino acids (M1-H431), with 4 glycosylation domains. BMP-7 Protein, Human (His) has a total length of 139 amino acids (S293-H431), is expressed in E. coli cells with a N-terminal 6*His-tag.

骨形态发生蛋白7（BMP-7）是一种多效性配体蛋白，属于TGFβ家族。BMP-7 参与调节癌细胞的增殖、侵袭和迁移，与多种人类肿瘤相关^[1]。
BMP-7 似乎还具有抗炎作用^[3]。
BMP-7 可以减轻血管钙化，还可以纠正与尿毒症相关的高磷酸盐血症，并刺激原位骨骼磷酸盐沉积，同时预防通过直接作用于血管平滑肌细胞来促进血管钙化^[4]。
BMP/TGFβ 信号传导参与血管和瓣膜稳态，这是胚胎发育的关键过程^[5]。
BMP-7 抑制原代人主动脉平滑肌细胞 (SMC) 由于血清、血小板衍生生长因子亚基 BB (PDGF-BB) 或 TGFβ1 刺激而增殖，并维持血管 SMC 表型^[3]。
BMP/TGFβ 信号传导可被包括 SMAD6 和 SMAD7 在内的抑制性 SMAD 终止，这些 SMAD 被 BMP 信号传导激活和诱导，并通过多种途径关闭 BMP 信号传导。机制^[2]。
然而，BMP-7敲低导致p-Smad1/5/9表达显着降低，并伴有BMP-7-Smad1/5/9信号通路失活上皮间质转化 (EMT) 过程逆转^[1]。
在肺癌中，BMP-7 抑制骨转移，并诱导细胞凋亡和细胞周期停滞。在恶性黑色素瘤中，BMP-7可以诱导间充质-上皮转化并抑制癌细胞的转移。BMP-7抑制上皮间质转化（EMT）相关基因和细胞侵袭，抑制端粒酶，缩短端粒，诱导乳腺癌细胞衰老和凋亡。还发现 BMP-7 在骨转移性乳腺癌和前列腺癌模型中可以增加细胞增殖和迁移潜力^[1]。
BMP-7 广泛存在于不同的动物，而小鼠的序列与大鼠（100.00%）和人类（97.67%）高度相似。

BMP-7 (300 ng/mL) initiates a synchronous wave of differentiation occurred, characterized by flattened, enlarged cells with reduced proliferation^[6].
BMP-7 (50 ng/mL; 1, 3, 5, 7, and 17 d) induces SCG neurons dendritic growth^[7].

Recombinant human BMP-7 induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED₅₀ for this effect is <0.5 μg/mL, corresponding to a specific activity is 2×10³ units/mg.

• 
  Recombinant human BMP-7 induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED50 for this effect is 0.2189 μg/mL, corresponding to a specific activity is 4.568×10³ units/mg.

Human

E. coli

N-6*His

P18075 (S293-H431)

655  [NCBI]

STGSKQRSQNRSKTPKNQEALRMANVAENSSSDQRQACKKHELYVSFRDLGWQDWIIAPEGYAAYYCEGECAFPLNSYMNATNHAIVQTLVHFINPETVPKPCCAPTQLNAISVLYFDDSSNVILKKYRNMVVRACGCH

Approximately 17 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, 500 mM arginine, pH 8.0 or 0.1% TFA, 20% Acetonitrile, pH 2.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Sun R, et al. Expression of BMP-7 in cervical cancer and inhibition of epithelial‑mesenchymal transition by BMP-7 knockdown in HeLa cells. Int J Mol Med. 2020 May;45(5):1417-1424.  [Content Brief]

  [2]. Miyazawa K, et al. Regulation of TGF-β Family Signaling by Inhibitory Smads. Cold Spring Harb Perspect Biol. 2017 Mar 1;9(3):a022095.  [Content Brief]

  [3]. Dorai H, et al. Bone morphogenetic protein-7 (osteogenic protein-1) inhibits smooth muscle cell proliferation and stimulates the expression of markers that are characteristic of SMC phenotype in vitro. J Cell Physiol. 2000 Jul;184(1):37-45.  [Content Brief]

  [4]. Mathew S, et al. Function and effect of bone morphogenetic protein-7 in kidney bone and the bone-vascular links in chronic kidney disease. Eur J Clin Invest. 2006 Aug;36 Suppl 2:43-50.  [Content Brief]

  [5]. Yang P, et al. The role of bone morphogenetic protein signaling in vascular calcification. Bone. 2020 Dec;141:115542.  [Content Brief]

  [6]. Xu RH, et al. BMP4 initiates human embryonic stem cell differentiation to trophoblast. Nat Biotechnol. 2002 Dec;20(12):1261-4.  [Content Brief]

  [7]. Beck HN, et al. Bone morphogenetic protein-5 (BMP-5) promotes dendritic growth in cultured sympathetic neurons. BMC Neurosci. 2001;2:12.  [Content Brief]