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  4. BOLA1 蛋白, Human (His)

BOLA1 蛋白, Human (His)

目录号: HY-P75596
产品使用指南

BOLA1 蛋白在组装线粒体铁硫 (Fe-S) 簇、促进其插入特定线粒体蛋白中发挥着至关重要的作用。它可能在此过程中与单硫醇谷氧还蛋白 GLRX5 合作，强调其在线粒体 Fe-S 簇生物发生中的作用。此外，BOLA1 可能有助于细胞防御氧化应激，突显线粒体铁稳态与氧化挑战反应之间的潜在联系。BOLA1 蛋白, Human (His) 是重组的 BOLA1 蛋白，由 E. coli 表达，带有 N-His 标签。BOLA1 蛋白, Human (His) 全长 137 个氨基酸，分子量约为 ~10 kDa。

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描述

BOLA1 Protein plays a vital role in assembling mitochondrial iron-sulfur (Fe-S) clusters, facilitating their insertion into specific mitochondrial proteins. It likely collaborates with monothiol glutaredoxin GLRX5 in this process, emphasizing its role in mitochondrial Fe-S cluster biogenesis. Additionally, BOLA1 may contribute to cellular defense against oxidative stress, highlighting potential connections between mitochondrial iron homeostasis and responses to oxidative challenges. BOLA1 Protein, Human (His) is the recombinant human-derived BOLA1 protein, expressed by E. coli, with N-His labeled tag. The total length of BOLA1 Protein, Human (His) is 137 a.a., with molecular weight of ~10 kDa.
研究背景

BOLA1 蛋白是线粒体铁硫 (Fe-S) 簇组装的关键因素，有助于将这些簇插入特定的线粒体蛋白中。在此过程中，它可能与单硫醇谷氧还蛋白 GLRX5 合作，强调了它在线粒体 Fe-S 簇生物合成中的作用。此外，BOLA1 可能有助于细胞防御氧化应激。它与 GLRX5 的相互作用进一步强调了它参与复杂的分子机制，强调了线粒体铁稳态和细胞对氧化挑战的反应之间的潜在联系。
种属

Human
表达系统

E. coli
标签

N-His
蛋白编号

Q9Y3E2 (M1-P137)
基因 ID
蛋白结构
N-term
His
BOLA1 (M1-P137)
Accession # Q9Y3E2
C-term
同用名
BolA-like protein 1; hBolA; BOLA1; CGI-143
分子量

Approximately 10 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

BOLA1 Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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