BRD4 蛋白, Human (His-Flag)

The BRD4 protein is a chromatin reader that recognizes and binds acetylated histones, transmits epigenetic memory and regulates transcription during cell division. It associates with acetylated chromatin throughout the cell cycle, maintaining chromatin structure and contributing to epigenetic memory. BRD4 Protein, Human (His-Flag) is the recombinant human-derived BRD4 protein, expressed by E. coli , with N-10*His, N-Flag labeled tag.

BRD4 蛋白充当染色质读取器，特异性识别和结合乙酰化组蛋白，从而在跨细胞分裂传递表观遗传记忆和调节转录方面发挥着至关重要的作用。它在整个细胞周期中始终与乙酰化染色质相关，保留乙酰化染色质状态并维持高阶染色质结构，有助于有丝分裂后 G1 基因转录的表观遗传记忆。在间期期间，BRD4 通过与 P-TEFb 复合物结合并将其招募到启动子来调节信号诱导基因的转录。此外，它与 JMJD6 合作，将 P-TEFb 复合物募集到远端增强子（称为抗暂停增强子），取代负调节子并将 P-TEFb 转化为可以磷酸化 RNA 聚合酶 II C 端结构域的活性形式。BRD4 还充当非典型蛋白激酶，促进 RNA 聚合酶 II C 末端结构域“Ser-2”的磷酸化。除了组蛋白之外，BRD4 还识别并结合乙酰化 RELA，促进 P-TEFb 复合物的募集并激活 NF-kappa-B。此外，它还充当 p53/TP53 介导的转录的调节剂，在 CK2 磷酸化后被招募到特定的靶启动子。在 DNA 损伤反应途径中，BRD4 充当染色质绝缘体，通过将 condensin-2 复合物募集到乙酰化组蛋白上并限制组蛋白 H2AX/H2A.x 磷酸化的扩散来抑制 DNA 损伤反应信号传导。这些不同的功能强调了 BRD4 在表观遗传调控、转录控制和染色质结构维护中的多功能作用。

Measured in a cell proliferation assay using M-NFS-60 cells. The ED₅₀ for this effect is ≤13.78 ng/mL, corresponding to a specific activity is ≥7.26×10⁴ units/mg.

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  Measured in a cell proliferation assay using M-NFS-60 cells. The ED₅₀ for this effect is 13.78 ng/mL, corresponding to a specific activity is 7.26×10⁴ units/mg.

Human

E. coli

N-10*His;N-Flag

O60885-1 (E49-E460)

23476  [NCBI]

ETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGTAKPGVSTVPNTTQASTPPQTQTPQPNPPPVQATPHPFPAVTPDLIVQTPVMTVVPPQPLQTPPPVPPQPQPPPAPAPQPVQSHPPIIAATPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLPPEPKTTKLGQRRESSRPVKPPKKDVPDSQQHPAPEKSSKVSEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLEAREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDE

Approximately 54-60 kDa

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  Greater than 90% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 200 mM NaCl, 1 mM DTT, 10% Glycerol, pH 7.5 or 50 mM HEPES, 300 mM NaCl, 1 mM DTT, 10% Glycerol, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.