1. 重组蛋白
  2. Immune Checkpoint Proteins
  3. Butyrophilins
  4. BTN3A1/CD277
  5. BTN3A1/CD277 蛋白, Human (HEK293)

BTN3A1/CD277 蛋白, Human (HEK293)

目录号: HY-P76179
COA 产品使用指南

BTN3A1/CD277 蛋白作为同二聚体,调节激活的 T 细胞增殖,控制细胞因子释放,并介导 T 细胞对磷酸化代谢物(例如异戊烯焦磷酸)升高的细胞的反应,有助于 T 细胞激活和适应性免疫反应。BTN3A1/CD277 蛋白, Human (HEK293) 是重组的 BTN3A1/CD277 蛋白,由 HEK293 表达,不带标签。

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     可免费申领三个不同产品的试用装。

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规格 价格 是否有货 数量
Free Sample   Apply now  
5 μg ¥620 In-stock
10 μg ¥1050 In-stock
50 μg ¥2940 In-stock
100 μg ¥5000 询价
> 100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

BTN3A1/CD277 Protein, functioning as a homodimer, regulates activated T-cell proliferation, controls cytokine release, and mediates T-cell response to cells with elevated phosphorylated metabolites, such as isopentenyl pyrophosphate, contributing to T-cell activation and the adaptive immune response. BTN3A1/CD277 Protein, Human (HEK293) is the recombinant human-derived BTN3A1/CD277 protein, expressed by HEK293 , with tag free.

研究背景

BTN3A1/CD277 蛋白参与与 T 细胞激活和适应性免疫反应相关的重要功能。它充当激活的 T 细胞增殖的调节剂,并控制这些细胞释放细胞因子和 IFNG。此外,它还介导 T 细胞对磷酸化代谢物(如异戊烯基焦磷酸)水平升高的感染和转化细胞的反应。BTN3A1/CD277 蛋白作为同二聚体发挥作用。

生物活性

Immobilized Human BTN3A1, No Tag at 0.5 μg/mL(100 μl/well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 8.8 ng/mL determined by ELISA.

种属

Human

表达系统

HEK293

标签

Tag Free

蛋白编号

O00481-1 (Q30-G254)

基因 ID
蛋白结构
N-term
BTN3A1 (Q30-G254)
Accession # O00481-1
C-term
同用名
Butyrophilin subfamily 3 member A1; CD277; BTN3A1; BTF5
分子量

26-32 kDa.

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

BTN3A1/CD277 Protein, Human (HEK293) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
BTN3A1/CD277 Protein, Human (HEK293)
目录号:
HY-P76179
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