C1QB 蛋白, Human (sf9, His)

The C1QB protein plays a critical role in the assembly of C1, the initial component of the serum complement system. C1q binds to the zymogens C1r and C1s, resulting in C1 assembly. C1QB Protein, Human (sf9, His) is the recombinant human-derived C1QB protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of C1QB Protein, Human (sf9, His) is 226 a.a., with molecular weight of ~30 kDa.

C1QB 蛋白在 C1（血清补体系统的初始成分）的形成中起着至关重要的作用。C1q 与酶原 C1r 和 C1s 结合，导致 C1 的组装。C1q 的胶原蛋白样区域与 Ca(2+) 依赖性 C1r(2)C1s(2) 酶原复合物相互作用，C1q 的球状头与 IgG 或 IgM 的 Fc 区域接合后，C1 就会被有效激活免疫复合物内的抗体。活性形式的 C1 构成钙依赖性三分子复合物，由摩尔比为 1:2:2 的 C1q、C1r 和 C1s 组成。C1q 亚组分由九个亚基组成，其中六个是 A 链和 B 链的二硫键连接的二聚体，其余三个形成 C 链的二硫键连接的二聚体。

Human

Sf9 insect cells

C-His

P02746 (Q28-A253)

713  [NCBI]

Approximately 30 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, 10% Glycerol, 0.5 mM TCEP, pH 7.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.