C2/Complement C2 蛋白, Mouse (HEK293, His)

Complement C2 is a component of the classical pathway of the complement system and is cleaved by activated factor C1, resulting in the formation of two distinct fragments: C2b and C2a. The subsequent serine protease activity of C2a is critical for its subsequent interaction with the complement factor C4b, leading to the formation of C3 or C5 convertase. C2/Complement C2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived C2/Complement C2 protein, expressed by HEK293 , with C-His labeled tag.

补体 C2 是补体系统内经典途径的重要组成部分，被激活的因子 C1 裂解成两个片段：C2b 和 C2a。生成的 C2a 片段具有丝氨酸蛋白酶活性，随后与补体因子 C4b 结合，最终形成 C3 或 C5 转化酶。这种酶复合物在补体系统的激活级联中发挥着关键作用，促进补体因子 C3 和 C5 的下游蛋白水解裂解，这是免疫反应中不可或缺的步骤。C2 在经典途径中精心策划的相互作用强调了其在介导免疫防御机制和维持免疫稳态方面的重要性。（参考：提供的资料）

Measured by its ability to cleave a colorimetric peptide 200 μM substrate Z-GR-SBzl, in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). that incubate at room temperature in kinetic mode for 5 minutes. The specific activity is 129.61 pmol/min/μg.

Mouse

HEK293

C-10*His

P21180-1 (A19-L760)

12263  [NCBI]

ALFCNQNVNITGGNFTLSHGWAPGSLLIYSCPLGRYPSPAWRKCQSNGQWLTPRSSSHHTLRSSRMVKAVCKPVRCLAPSSFENGIYFPRLVSYPVGSNVSFECEQDFTLRGSPVRYCRPNGLWDGETAVCDNGASHCPNPGISVGTARTGLNFDLGDKVRYRCSSSNMVLTGSAERECQSNGVWSGSEPICRQPYSYDFPEDVASALDTSLTNLLGATNPTQNLLTKSLGRKIIIQRSGHLNLYLLLDASQSVTEKDFDIFKKSAELMVERIFSFEVNVSVAIITFASQPKTIMSILSERSQDVTEVITSLDSASYKDHENATGTNTYEVLIRVYSMMQSQMDRLGMETSAWKEIRHTIILLTDGKSNMGDSPKKAVTRIRELLSIEQNRDDYLDIYAIGVGKLDVDWKELNELGSKKDGERHAFILQDAKALQQIFEHMLDVSKLTDTICGVGNMSANASDQERTPWQVTFKPKSKETCQGSLISDQWVLTAAHCFHDIQMEDHHLWRVNVGDPTSQHGKEFLVEDVIIAPGFNVHAKRKQGISEFYADDIALLKLSRKVKMSTHARPICLPCTVGANMALRRSPGSTCKDHETELLSQQKVPAHFVALNGNRLNINLRTGPEWTRCIQAVSQNKNIFPSLTNVSEVVTDQFLCSGMEEEDDNPCKGESGGAVFLGRRYRFFQVGLVSWGLFDPCHGSSNKNLRKKPPRGVLPRDFHISLFRLQPWLRQHLDGVLDFLPL

Approximately 90-110 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.