CA125 蛋白, Human (Biotinylated, HEK293, His-Avi)

The CA125 protein acts by binding to MSLN and plays a crucial role in forming a protective and lubricating barrier on mucosal surfaces. This interaction promotes heterotypic cell adhesion and is suggested to play an important role in peritoneal metastasis of ovarian cancer. CA125 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived CA125 protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of CA125 Protein, Human (Biotinylated, HEK293, His-Avi) is 264 a.a., with molecular weight of 52-80 kDa.

CA125 蛋白被认为在建立针对粘膜表面颗粒和感染因子的保护和润滑屏障方面发挥着至关重要的作用。它通过与 MSLN 结合发挥其功能，其中相互作用介导异型细胞粘附。这种粘附特性可能在卵巢癌腹膜转移中发挥重要作用，因为 CA125 通过与 MSLN 结合启动细胞附着到间皮上皮。CA125 和 MSLN 之间的相互作用强调了它可能参与与癌症转移相关的粘附事件，特别是在卵巢癌进展到腹膜腔的情况下。

Immobilized Human MSLN, hFc Tag at 2 μg/mL (100 μl/well) on the plate. Dose response curve for Biotinylated Human CA125, His Tag with the EC₅₀ of 0.24 μg/mL determined by ELISA.

Human

HEK293

C-Avi;C-His

Q8WXI7 (G12660-M12923)

94025  [NCBI]

52-80 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.