Cadherin-1/CD324 蛋白, Mouse (HEK293, C-His)

Cadherin-1/CD324 is a calcium-dependent cell adhesion protein that orchestrates homogeneous interactions, promoting cell cohesion and potential cell type sorting. CDH1 regulates epithelial cell adhesion, mobility, and proliferation and acts as an invasive suppressor. Cadherin-1/CD324 Protein, Mouse (HEK293, C-His) is the recombinant mouse-derived Cadherin-1/CD324 protein, expressed by HEK293 , with C-6*His labeled tag.

Cadherin-1，也称为 CD324，代表一组钙依赖性细胞粘附蛋白，表现出优先的同质相互作用，促进连接细胞的凝聚力，并可能有助于异质细胞类型的分选。CDH1 复杂地参与细胞间粘附、移动性和上皮细胞增殖的调节，作为侵袭性抑制因子发挥着重要作用。除了其粘附功能外，CDH1 还充当整合素 α-E/β-7 的配体，表明其参与多种细胞过程。值得注意的是，E-Cadherin/CTF2（CDH1 的裂解产物）有助于 Abeta 前体的非淀粉样蛋白降解，对 APP C99 和 C83 的产生产生强烈的抑制作用。

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  Measured by its ability of the immobilized protein to support the adhesion of the MCF-7 human breast cancer. The ED₅₀ is < 0.3165 μg/mL, corresponding to a specific activity of >3.1 × 10³ units/mg.

Mouse

HEK293

C-6*His

P09803 (D157-V709)

12550  [NCBI]

DWVIPPISCPENEKGEFPKNLVQIKSNRDKETKVFYSITGQGADKPPVGVFIIERETGWLKVTQPLDREAIAKYILYSHAVSSNGEAVEDPMEIVITVTDQNDNRPEFTQEVFEGSVAEGAVPGTSVMKVSATDADDDVNTYNAAIAYTIVSQDPELPHKNMFTVNRDTGVISVLTSGLDRESYPTYTLVVQAADLQGEGLSTTAKAVITVKDINDNAPVFNPSTYQGQVPENEVNARIATLKVTDDDAPNTPAWKAVYTVVNDPDQQFVVVTDPTTNDGILKTAKGLDFEAKQQYILHVRVENEEPFEGSLVPSTATVTVDVVDVNEAPIFMPAERRVEVPEDFGVGQEITSYTAREPDTFMDQKITYRIWRDTANWLEINPETGAIFTRAEMDREDAEHVKNSTYVALIIATDDGSPIATGTGTLLLVLLDVNDNAPIPEPRNMQFCQRNPQPHIITILDPDLPPNTSPFTAELTHGASVNWTIEYNDAAQESLILQPRKDLEIGEYKIHLKLADNQNKDQVTTLDVHVCDCEGTVNNCMKAGIVAAGLQV

Approximately 82 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.