Cadherin-1/CD324 蛋白, Rat (HEK293, His)

Cadherin-1/CD324 protein, a calcium-dependent adhesion molecule, mediates cell processes. It interacts homophilically with other cadherin molecules, sorting and organizing cell types. CDH1 regulates adhesion, mobility, and proliferation of epithelial cells. It serves as an integrin ligand, suppressing invasion and promoting non-amyloidogenic degradation. CDH1 inhibits APP C99 and C83 production. Cadherin-1/CD324 Protein, Rat (HEK293, His) is the recombinant rat-derived Cadherin-1/CD324 protein, expressed by HEK293 , with C-His labeled tag.

Cadherin-1/CD324 蛋白是一种钙依赖性细胞粘附分子，在各种细胞过程中发挥着关键作用。通过优先以同种方式与相同类型的其他钙粘蛋白分子相互作用，CDH1 有助于组织内不同细胞类型的分类和组织。此外，它还参与调节上皮细胞的细胞间粘附、移动性和增殖。CDH1 还充当整合素 α-E/β-7 的配体，并在侵袭中表现出有效的抑制作用。此外，它还能促进 Abeta 前体的非淀粉样蛋白降解，并对 APP C99 和 C83 的产生产生强烈的抑制作用。

Measured by the ability of the immobilized protein to support the adhesion of MCF-7 cells. When 5×10⁴cells/well are added to Rat Cadherin-1/CD324 Protein, cell adhesion is enhanced in a dose dependent manner after 30 minutes at 37℃. The ED₅₀ for this effect is 0.3603 μg/mL, corresponding to a specific activity is 2.775×10³ U/mg.

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  Measured by the ability of the immobilized protein to support the adhesion of MCF-7 cells. When 5×10⁴ cells/well are added to Rat Cadherin-1/CD324 Protein, cell adhesion is enhanced in a dose dependent manner after 30 minutes at 37℃. The ED₅₀ for this effect is 0.3603 μg/mL, corresponding to a specific activity is 2.775×10³ U/mg.

Rat

HEK293

C-10*His

Q9R0T4 (Q24-A713)

83502  [NCBI]

QPESESDSCRPGFSSEVYTFLVPERHLERGHILGRVKFEGCTGRPRTAFFSEDSRFKVSTDGVITVKRHLKLHKLETSFLVHAWDSSYRKLSTKVTLKSLGHHHHRHHHRDPVSESNPELLTFPSFHQGLRRQKRDWVIPPINCPENQKGEFPQRLVQIKSNRDKETTVFYSITGPGADKPPVGVFIIERETGWLKVTQPLDREAIDKYLLYSHAVSSNGEAVEDPMEIVVTVTDQNDNRPEFIQEVFEGSVAEGALPGTSVMQVSATDADDDINTYNAAIAYTILSQDPELPHKNMFTVNRDTGVISVVTSGLDRESYPTYTLVVQAADLQGEGLSTTAKAVITVKDINDNAPIFNPSTYQGQVLENEVGARIATLKVTDDDAPNTPAWNAVYTVVNDPDHQFTVITDPKTNEGILKTAKGLDFEAKQQYILHVTVENEEPFEGSLVPSTATVTVDVVDVNEAPIFVPAEKRVEVPEDFGVGLEIASYTAREPDTFMEQKITYRIWRDTANWLEINPETGVISTRAEMDREDSEHVKNSTYTALIIATDDGSPIATGTGTLLLVLSDVNDNAPIPEPRNMQFCQRNPKPHVITILDPDLPPNTSPFTAELTHGASVNWTIEYNDAEQESLILQPRKDLEIGEYKINLKLSDNQNKDQVTTLEVHVCDCEGTVNNCMKAISLEAGLQVPA

Approximately 80 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.