Cadherin-13 蛋白, Mouse (HEK293, His)

Cadherin-13 proteins are calcium-dependent cell adhesion molecules that exhibit a preference for homogeneous interactions, promote connections between adjacent cells, and may play a role in cell type sorting. Cadherin-13 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Cadherin-13 protein, expressed by HEK293 , with C-His labeled tag. The total length of Cadherin-13 Protein, Mouse (HEK293, His) is 692 a.a., with molecular weight of 90-110 kDa.

Cadherin-13 是一种钙依赖性细胞粘附蛋白，与经典钙粘蛋白相比，因其独特的功能而脱颖而出。与典型的钙粘蛋白 EC1 结构域链交换同二聚化机制不同，Cadherin-13 通过独特的亲同性粘附界面实现这一点，连接两个靠近 Ca2+ 结合位点的细长 EC1-EC2 结构域。这种排列形成四面体，X状形状，展示了不同的同二聚化模式。该蛋白质可能充当神经细胞生长的负调节因子，并且其以同质方式优先与其自身相互作用的能力表明其在神经组织内异质细胞类型的分选中发挥作用。

Measured by the ability of the immobilized protein to inhibit the adhesion of HUVEC human umbilical vein endothelial cells. When 5 x 10⁴ cells/well are added to Recombinant Mouse Cadherin-13 coated plates, cell adhesion is inhibited in a dose dependent manner after 2.5 hours at 37 °C. The ED₅₀ for this effect is 4.025 μg/mL, corresponding to a specific activity is 248.447 units/mg.

• 
  Measured by the ability of the immobilized protein to inhibit the adhesion of HUVEC human umbilical vein endothelial cells. . When 5 x 10⁴ cells/well are added to Recombinant Mouse Cadherin-13 coated plates, cell adhesion is inhibited in a dose dependent manner after 2.5 hours at 37 °C. The ED₅₀ for this effect is 4.025 μg/mL, corresponding to a specific activity is 248.447 units/mg.

Mouse

HEK293

C-10*His

Q9WTR5 (M1-A692)

12554  [NCBI]

DDLECTPGFQRKVLHIHQPAEFIEDQPVLNLTFNDCKGNEKLHYEVSSPHFKVNSDGTLVALRNITAVGRTLFVHARTPHAEDMAELVIVGGKDIQGSLQDIFKFARTSPVPRQKRSIVVSPILIPENQRQPFPRDVGKVVDSDRPEGSKFRLTGKGVDQDPKGTFRINENTGSVSVTRTLDRETIATYQLYVETTDASGKTLEGPVPLEVIVIDQNDNRPIFREGPYIGHVMEGSPTGTTVMRMTAFDADDPATDNALLRYNIRQQTPDKPSPNMFYIDPEKGDIVTVVSPALLDRETLENPKYELIIEAQDMAGLDVGLTGTATATIVIDDKNDHSPKFTKKEFQATVEEGAVGVIVNLTVEDKDDPTTGAWRAAYTIINGNPGQSFEIHTNPQTNEGMLSVVKPLDYEISAFHTLLIKVENEDPLVPDVSYGPSSTATVHITVLDVNEGPVFYPDPMMVTKQENISVGSVLLTVNATDPDSLQHQTIRYSIYKDPAGWLSINPINGTVDTTAVLDRESPFVHNSVYTALFLAIDSGNPPATGTGTLLITLEDINDNAPVIYPTVAEVCDDARNLSVVILGASDKDLHPNTDPFKFEIHKQTVPDKVWKISKINNTHALVSLLQNLNKANYNLPIMVTDSGKPPMTNITDLRVQVCSCKNSKVDCNGA

Approximately 90-110 kDa due to the glycosylation.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.