Cadherin-13 蛋白, Rat (HEK293)

Cadherin-13 Protein is an atypical member of the cadherin family, devoid of a transmembrane domain and anchored to the exterior surface of the plasma membrane via a glycosylphosphatidylinositol anchor. And it exhibits a preference for homophilic interactions, fostering connections between neighboring cells and potentially playing a role in cell type sorting. Cadherin-13 may act as negative regulators of neural cell growth. Moreover, it may act rather as a signalling receptor participating in recognition of the environment and regulation of cell motility, proliferation and phenotype. Cadherin-13 Protein, Rat (HEK293) is the recombinant rat-derived Cadherin-13 protein, expressed by HEK293 , with tag free.

Cadherin-13 是钙粘蛋白家族的非典型成员，缺乏跨膜结构域，通过糖基磷脂酰肌醇锚定物锚定在质膜的外表面。它表现出对同质相互作用的偏好，促进相邻细胞之间的联系，并可能在细胞类型分选中发挥作用。Cadherin-13 可能充当神经细胞生长的负调节因子。Cadherin-13 在神经和心血管系统 (内皮细胞和平滑肌细胞) 中高度表达。因此，它可能促进增殖性血管疾病的进展，例如动脉粥样硬化、再狭窄和肿瘤血管生成。此外，它可能充当信号传导受体，参与环境识别和细胞运动、增殖和表型的调节。PI3K/Akt/GSK3β 信号轴的 Cadherin-13 依赖性激活可保护内皮细胞免受氧化应激诱导的细胞凋亡^[1][2]。

Measured by the ability of the immobilized protein to inhibit the adhesion of HUVEC human umbilical vein endothelial cells. The ED₅₀ for this effect is 4.702 μg/mL, corresponding to a specific activity is 212.67 units/mg.

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  Measured by the ability of the immobilized protein to inhibit the adhesion of HUVEC human umbilical vein endothelial cells. The ED₅₀ for this effect is 4.702 μg/mL, corresponding to a specific activity is 212.67 units/mg

Rat

HEK293

Tag Free

Q8R490 (A22-A692)

192248  [NCBI]

AGDLDCTPGFQQKVLHIHQPAEFIEDQPVLNLTFSDCKGNKKLHYEVSSPYFKVNSDGTLVAVRNITAVGRTLFVHARTPHAEDMAELVIVGGKDIQGSLQDIFKFARTSPVPRQKRSIVVSPILIPENQRQPFPRDVGKVVDSDRPEGSKFRLTGKGVDQDPKGTFRINENTGSVSVTRTLDRETIATYQLFVETTDASGRTLEGPVPLEVIVIDQNDNRPIFREGPYIGHVMEGSPTGTTVMRMTAFDADDPATDNALLRYNIRQQTPDKPSPNMFYIDPEKGDIVTVVSPALLDRETLENPKYELIIEAQDMAGLDVGLTGTATATIVIDDKNDHSPKFTKKEFQATVEEGAVGVIVNLTVEDKDDPATGAWRAAYTIINGNPGQSFEIHTNPQTNEGMLSVVKPLDYEISAFHTLLIKVENEDPLVPDVSYGPSSTATVHITVLDVNEGPVFYPDPMMVTKQENISVGSVLLTVNATDPDSLQHQTIRYSVYKDPAGWLSINPINGTVDTTAVLDRESPFVHNSVYTALFLAIDSGNPPATGTGTLMITLEDVNDNAPVIYPTVAEVCDDARNLSVVILGASDKDLHPNTDPFKFEIHKQTVPDKVWKISKINNTHALVSLLQNLNKANYNLPIMVTDSGKPPMTNITDLRVQVCSCKNSKVDCNAA

Approximately 95 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.