2. 重组蛋白
  3. Receptor Proteins
  4. Cell Adhesion Molecules (CAMs)
  5. Cadherins
  6. K-Cadherin/Cadherin-6
  7. Cadherin-6/KCAD 蛋白, Human (594a.a, HEK293, His)

Cadherin-6/KCAD 蛋白, Human (594a.a, HEK293, His)

目录号: HY-P7703
产品使用指南

Cadherin-6 蛋白, Human (594a.a, HEK293, His)是一种由 HEK293 细胞表达的，N 末端有 His 标签的重组人 Cadherin-6。Cadherin-6 (CDH6) 是 2 型钙粘蛋白，在胚胎发育过程中会驱动上皮细胞向间充质状态 (EMT) 转分化，并且在癌症中异常重新激活。

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描述

Cadherin-6 Protein, Human (594a.a, HEK293, His), a recombinant human Cadherin-6 produced in HEK293 cells, has a His tag at the N-terminus. Cadherin-6 (CDH6) is a type 2 cadherin, which drives epithelial cells toward a mesenchymal condition (EMT) during embryonic development and it is aberrantly re-activated in cancer[1].
研究背景

Cadherin-6 (CDH6) 是一种 II 类钙粘蛋白，参与中枢神经系统和肾脏的形态发生。正如许多参与胚胎发生的蛋白质一样，CDH6 在癌症中异常重新激活[1]。
种属

Human
表达系统

HEK293
标签

C-6*His
蛋白编号

P55285 (T22-A615)
基因 ID
蛋白结构
N-term
CDH6 (T22-A615)
Accession # P55285
6*His
C-term
同用名
rHuCadherin-6, His; Cadherin-6; Kidney Cadherin; CDH6
氨基酸序列

TPLSKRTSGFPAKKRALELSGNSKNELNRSKRSWMWNQFFLLEEYTGSDYQYVGKLHSDQDRGDGSLKYILSGDGAGDLFIINENTGDIQATKRLDREEKPVYILRAQAINRRTGRPVEPESEFIIKIHDINDNEPIFTKEVYTATVPEMSDVGTFVVQVTATDADDPTYGNSAKVVYSILQGQPYFSVESETGIIKTALLNMDRENREQYQVVIQAKDMGGQMGGLSGTTTVNITLTDVNDNPPRFPQSTYQFKTPESSPPGTPIGRIKASDADVGENAEIEYSITDGEGLDMFDVITDQETQEGIITVKKLLDFEKKKVYTLKVEASNPYVEPRFLYLGPFKDSATVRIVVEDVDEPPVFSKLAYILQIREDAQINTTIGSVTAQDPDAARNPVKYSVDRHTDMDRIFNIDSGNGSIFTSKLLDRETLLWHNITVIATEINNPKQSSRVPLYIKVLDVNDNAPEFAEFYETFVCEKAKADQLIQTLHAVDKDDPYSGHQFSFSLAPEAASGSNFTIQDNKDNTAGILTRKNGYNRHEMSTYLLPVVISDNDYPVQSSTGTVTVRVCACDHHGNMQSCHAEALIHPTGLSTGAHHHHHH

分子量

Approximately 109.0 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)
参考文献

Cadherin-6/KCAD Protein, Human (594a.a, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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