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  3. CALML3 蛋白, Human (GST)

CALML3 蛋白, Human (GST)

目录号: HY-P72110
产品使用指南

CALML3 蛋白具有双重作用,充当非常规肌球蛋白 10 (MYO10) 的特定轻链并增强 MYO10 翻译。CALML3 具有潜在的分子伴侣作用,有助于新兴 MYO10 重链蛋白的合成。CALML3 蛋白, Human (GST) 是重组的 CALML3 蛋白,由 E. coli 表达,带有 N-GST 标签。CALML3 蛋白, Human (GST) 全长 149 个氨基酸,分子量约为 ~43.9 kDa。

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描述

The CALML3 protein has a dual role, acting as a specific light chain of unconventional myosin 10 (MYO10) and enhancing MYO10 translation. CALML3 has a potential molecular chaperone role and contributes to the synthesis of the emerging MYO10 heavy chain protein. CALML3 Protein, Human (GST) is the recombinant human-derived CALML3 protein, expressed by E. coli , with N-GST labeled tag. The total length of CALML3 Protein, Human (GST) is 149 a.a., with molecular weight of ~43.9 kDa.

研究背景

CALML3 蛋白似乎具有双重作用:充当非常规肌球蛋白 10 (MYO10) 的特定轻链并增强 MYO10 翻译。CALML3 可以作为潜在的分子伴侣,帮助新兴的 MYO10 重链蛋白的合成。此外,CALML3 表现出与钙调蛋白竞争结合细胞底物的能力,表明在钙依赖性信号通路中具有调节作用。特别是,它与 MYO10 的相互作用是钙依赖性的,并且证明对于 MYO10 在丝状伪足延伸中的功能至关重要,丝状伪足是细胞形态的关键过程。CALML3 和 MYO10 之间复杂的相互作用强调了其在调节细胞骨架动力学和细胞功能中的重要性,促使进一步研究这些相互作用背后的精确分子机制及其功能后果。

种属

Human

表达系统

E. coli

标签

N-GST

蛋白编号

P27482 (M1-K149)

基因 ID

810  [NCBI]

蛋白结构
N-term
GST
CALML3 (M1-K149)
Accession # P27482
C-term
同用名
CALL3_HUMAN; CALML3; Calmodulin like 3; Calmodulin related protein NB 1; Calmodulin-like protein 3; Calmodulin-related protein NB-1; CaM like protein; CaM-like protein; CLP; OTTHUMP00000019004
氨基酸序列

MADQLTEEQVTEFKEAFSLFDKDGDGCITTRELGTVMRSLGQNPTEAELRDMMSEIDRDGNGTVDFPEFLGMMARKMKDTDNEEEIREAFRVFDKDGNGFVSAAELRHVMTRLGEKLSDEEVDEMIRAADTDGDGQVNYEEFVRVLVSK

分子量

Approximately 43.9 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

CALML3 Protein, Human (GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CALML3 Protein, Human (GST)
目录号:
HY-P72110
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