Calreticulin/CALR 蛋白, Pig (P.pastoris, His)

Calreticulin (CALR) is an essential calcium-binding chaperone involved in ER functions. It interacts with glycoproteins, facilitates NR3C1 nuclear export, regulates maternal gene expression, and aids in oocyte maturation. CALR is released during oocyte activation and prevents polyspermy. It forms an EIF2 complex with CELF1/CUGBP1, CALR3, EIF2S1, EIF2S2, HSP90B1, and HSPA5 and interacts with PDIA3/ERp57, SPACA9, TRIM21, PPIB, PDIA5, and CLCC1. Calreticulin/CALR Protein, Pig (P.pastoris, His) is the recombinant pig-derived Calreticulin/CALR protein, expressed by P. pastoris , with N-His labeled tag.

钙网蛋白 (CALR) 是一种钙结合伴侣，在内质网 (ER) 中发挥着至关重要的作用，通过钙网蛋白/钙连接蛋白循环促进折叠、寡聚组装和质量控制。它与内质网中合成的几乎所有单葡萄糖基化糖蛋白瞬时相互作用。此外，CALR 与 NR3C1 的 DNA 结合域相互作用，促进其核输出。CALR 还参与调节母体基因表达，并可能通过维持钙稳态来促进卵母细胞成熟。在未激活的卵母细胞中，CALR 位于皮质颗粒中，并在卵母细胞激活时的皮质反应过程中释放，可能防止多精受精。CALR 以单体形式存在，是由 CELF1/CUGBP1、CALR、CALR3、EIF2S1、EIF2S2、HSP90B1 和 HSPA5 组成的 EIF2 复合体的一部分。它与多种蛋白质相互作用，例如 PDIA3/ERp57、SPACA9、TRIM21、NR3C1、PPIB、PDIA5 和 CLCC1。

Pig

P. pastoris

N-His

P28491 (E18-L417)

100381266  [NCBI]

EPTIYFKEQFLDGDGWTDRWIESKHKPDFGRFVLSSGKFYGDQEKDKGLQTSQDARFYALSARFEPFSNKGQTLVVQFTVKHEQNIDCGGGYVKLFPDGLDQTDMHGDSEYNIMFGPDICGPGTKKVHVIFNYKGKNVLINKDIRCKDDEFTHLYTLIVRPDNTYEVKIDNSQVESGSLEDDWDFLPPKKIKDPDAVKPEDWDERAKIDDPTDSKPEDWDKPEHIPDPDAKKPEDWDEEMDGEWEPPVIQNPEYKGEWKPRQIDNPDYKGTWIHPEIDNPEYSPDSNIYAYENFAVLGLDLWQVKSGTIFDNFLITNDEAYAEEFGNETWGVTKAAEKQMKDKQDEEQRLKEEEEEKKRKEEEEVDKEDEEDKDEDEEEEDEKEEEEEEDAAAGQAKDEL

Approximately 48.6 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.