Calsequestrin 1 蛋白, Human

Calsequestrin 1 protein is a high-capacity calcium-binding protein that serves as an internal calcium store in muscles. Calsequestrin 1 Protein, Human is the recombinant human-derived Calsequestrin 1 protein, expressed by E. coli , with tag free. The total length of Calsequestrin 1 Protein, Human is 362 a.a., with molecular weight of ~52 kDa.

Calsequestrin 1 是一种高容量钙结合蛋白，可作为肌肉中的内部钙储存。蛋白质表面（特别是亚基界面）的酸性残基簇有利于大约 80 个 Ca(2+) 离子的结合。该蛋白在通过钙释放通道 RYR1 调节腔内 Ca(2+) 的释放方面发挥着关键作用，从而有助于启动肌肉收缩。此外，Calsequestrin 1 还可作为钙池操纵的 Ca(2+) 内流 (SOCE) 活性的负调节剂。作为响应细胞内钙消耗而增加的单体存在，它还可以形成同二聚体和更高阶的同寡聚体，其中 Ca(2+) 离子促进寡聚化。Calsequestrin 1 与 STIM1 相互作用，优先以其单体形式相互作用，并且这种相互作用在细胞内钙耗尽后增强。此外，它与 ASPH 和 TRDN 相互作用，突显其参与与钙稳态相关的多种细胞过程。

Human

E. coli

Tag Free

P31415 (Q35-D396)

844  [NCBI]

QEGLDFPEYDGVDRVINVNAKNYKNVFKKYEVLALLYHEPPEDDKASQRQFEMEELILELAAQVLEDKGVGFGLVDSEKDAAVAKKLGLTEVDSMYVFKGDEVIEYDGEFSADTIVEFLLDVLEDPVELIEGERELQAFENIEDEIKLIGYFKSKDSEHYKAFEDAAEEFHPYIPFFATFDSKVAKKLTLKLNEIDFYEAFMEEPVTIPDKPNSEEEIVNFVEEHRRSTLRKLKPESMYETWEDDMDGIHIVAFAEEADPDGFEFLETLKAVAQDNTENPDLSIIWIDPDDFPLLVPYWEKTFDIDLSAPQIGVVNVTDADSVWMEMDDEEDLPSAEELEDWLEDVLEGEINTEDDDDDDDD

Approximately 52 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4, 10% Glycerol.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).