CaMKII alpha/CAMK2A 蛋白, Human (sf9, GST)

CaMKII α/CAMK2A protein functions autonomously upon Ca(2+)/calmodulin binding and autophosphorylation. CaMKII alpha/CAMK2A Protein, Human (sf9, GST) is the recombinant human-derived CaMKII alpha/CAMK2A protein, expressed by Sf9 insect cells , with N-GST labeled tag. The total length of CaMKII alpha/CAMK2A Protein, Human (sf9, GST) is 478 a.a., with molecular weight of ~80 kDa.

CaMKII α/CAMK2A 蛋白是一种钙/钙调蛋白依赖性激酶，在 Ca(2+)/钙调蛋白结合和自磷酸化后自主发挥作用，在突触可塑性、神经递质释放和长时程增强等各种细胞过程中发挥着至关重要的作用。作为兴奋性突触中 NMDAR 信号复合体的成员，它调节 AMPAR 的 NMDAR 依赖性增强，从而影响兴奋性突触传递。CaMKII α 还有助于树突棘发育并调节发育中神经元的迁移。除了这些功能之外，它还磷酸化转录因子 FOXO3 以激活其转录活性，并响应钙信号传导而靶向转录因子 ETS1，从而降低 ETS1 对 DNA 的亲和力。响应干扰素-γ (IFN-γ) 刺激，它催化 STAT1 磷酸化，从而刺激 JAK-STAT 信号通路。此外，响应干扰素-β (IFN-β) 刺激，CaMKII α 会刺激 JAK-STAT 信号通路，并通过调节 DAGLA 活性，充当 2-花生四烯酰甘油 (2-AG) 介导的突触信号传导的负调节因子。

The specific activity was determined to be 160 nmol/min/mg using Autocamtide-2 synthetic peptide (KKALRRQETVDAL-amide) as substrate.

Human

Sf9 insect cells

N-GST

Q9UQM7-1 (M1-H478)

815  [NCBI]

Approximately 80 kDa

Greater than 85% as determined by reducing SDS-PAGE

溶液.

Supplied as a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, 0.5 mM PMSF, 0.5 mM Reduced Glutathione, pH 8.0

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.