CANT1 蛋白, Human (HEK293, His)

CANT1 is a calcium-dependent nucleotidase that shows a clear preference for UDP, and the order of its enzymatic activity is UDP > GDP > UTP > GTP. It is worth noting that it has little activity on ADP and even less activity on ATP, but it does not hydrolyze AMP and GMP. CANT1 Protein, Human (HEK293, His) is the recombinant human-derived CANT1 protein, expressed by HEK293 , with N-His labeled tag.

CANT1是一种钙依赖性核苷酸酶，对UDP作为底物表现出强烈的偏好，其活性顺序为UDP > GDP > UTP > GTP。值得注意的是，它对 ADP 的活性非常低，对 ATP 的活性甚至更低，但它不会水解 AMP 和 GMP。这种酶谱表明在核苷酸代谢中具有特定作用。CANT1 参与蛋白聚糖合成，表明它参与与这些重要大分子的修饰和调节相关的过程。CANT1 的钙依赖性性质凸显了其通过细胞内钙水平的调节，为其酶功能和潜在的细胞作用增加了一层额外的复杂性。

Measured by its ability to hydrolyze the beta 5'-phosphate group from the substrate uridine-5'-diphosphate (UDP) that incubate at room temperature for 20 min at 37°C. The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit. The specific activity is 285089.234 pmol/min/μg.

Human

HEK293

N-6*His

Q8WVQ1-1 (G80-I401)

124583  [NCBI]

GQAPANWYNDTYPLSPPQRTPAGIRYRIAVIADLDTESRAQEENTWFSYLKKGYLTLSDSGDKVAVEWDKDHGVLESHLAEKGRGMELSDLIVFNGKLYSVDDRTGVVYQIEGSKAVPWVILSDGDGTVEKGFKAEWLAVKDERLYVGGLGKEWTTTTGDVVNENPEWVKVVGYKGSVDHENWVSNYNALRAAAGIQPPGYLIHESACWSDTLQRWFFLPRRASQERYSEKDDERKGANLLLSASPDFGDIAVSHVGAVVPTHGFSSFKFIPNTDDQIIVALKSEEDSGRVASYIMAFTLDGRFLLPETKIGSVKYEGIEFI

Approximately 36-40 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.