1. 重组蛋白
  2. Enzymes & Regulators
  3. Lyases (EC 4) Carbonic Anhydrase
  4. Carbonic Anhydrase 12 (CA-XII)
  5. Carbonic Anhydrase 12 蛋白, Mouse (HEK293, His)

Carbonic Anhydrase 12 蛋白, Mouse (HEK293, His)

目录号: HY-P7716
产品使用指南

Carbonic Anhydrase 12 蛋白, Mouse (HEK293, His)是一种由 HEK293 细胞表达的,N 末端有 His 标签的重组鼠 Carbonic Anhydrase 12。低氧性肿瘤过表达两种 carbonic anhydrases (CA),CA IX 和 XII,涉及与肿瘤发生有关的复杂过程 (pH 调节,肿瘤的代谢,侵袭和扩散)。

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描述

Carbonic Anhydrase 12 Protein, Mouse (HEK293, His), a recombinant Mouse Carbonic Anhydrase 12 produced in HEK293 cells, has a His tag at the N-terminus. Hypoxic tumors overexpress two carbonic anhydrases (CA), CA IX and XII, involved in complex processes connected to tumorigenesis (pH regulation, metabolism, invasion, and dissemination of the tumor)[1].

研究背景

CA 在人类中广泛存在,迄今为止已知有 12 种催化活性亚型和 3 种无催化亚型(CA VIII、X 和 XI)。许多具有催化活性的CA具有作为可逆CO2水合为碳酸氢盐和质子的催化剂的优异活性,是自然界中已知的最有效的酶之一。CA IX 和 XII 已被验证为抗肿瘤/抗转移药物靶点,可用于缺氧肿瘤的成像[1]

种属

Mouse

表达系统

HEK293

标签

C-6*His

蛋白编号

Q8CI85 (A25-S301)

基因 ID
蛋白结构
N-term
Ca12 (A25-S301)
Accession # Q8CI85
6*His
C-term
中文名
重组小鼠碳酸酐酶 12, His (HEK293 表达)
同用名
rMuCarbonic Anhydrase 12, His; Carbonic anhydrase 12; Carbonate dehydratase XII; CA-XII; CA12; Carbonate dehydratase XII;
氨基酸序列

APLNGSKWTYVGPAGEKNWSKKYPSCGGLLQSPIDLHSDILQYDASLAPLQFQGYNVSVEKLLNLTNDGHSVRLNLNSDMYIQGLQPHHYRAEQLHLHWGNRNDPHGSEHTVSGKHFAAELHIVHYNSDLYPDFSTASDKSEGLAVLAVLIEIGSANPSYDKIFSHLQHVKYKGQQVLIPGFNIEELLPESPGEYYRYEGSLTTPPCYPTVLWTVFRNPVQISQEQLLALETALYFTHMDDPTPREMINNFRQVQKFDERLVYISFRQGLLTDTGLSHHHHHH

分子量

35-40 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
参考文献

Carbonic Anhydrase 12 Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Carbonic Anhydrase 12 Protein, Mouse (HEK293, His)
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