Carbonic Anhydrase 3 蛋白, Human (His)

Carbonic Anhydrase 3 Protein, Human (His) expresses in E. coli with a His tag at the N-terminus. Carbonic anhydrase 3 (CA III) is a zinc metalloenzyme known to catalyze the reversible hydration of CO₂ to HCO₃^-[1].

碳酸酐酶 III (CAIII) 是一种代谢酶，也是细胞内 pH 值的调节剂。CAIII 是一种约 30 kDa 的胞质蛋白，在肝脏、脂肪细胞和骨骼肌中高水平存在。它是 CA 同工酶中活性较低的酶，但对大多数磺酰胺抑制剂具有耐药性。CAIII 可能促进糖酵解中间体快速转化为草酰乙酸和柠檬酸盐，并刺激它们掺入脂肪酸^[2]。

Measured by its esterase activity.The specific activity is >5 pmoles/min/μg.

Human

E. coli

C-His

NP_005172.1 (A1-K260)

761  [NCBI]

MAKEWGYASHNGPDHWHELFPNAKGENQSPVELHTKDIRHDPSLQPWSVSYDGGSAKTILNNGKTCRVVFDDTYDRSMLRGGPLPGPYRLRQFHLHWGSSDDHGSEHTVDGVKYAAELHLVHWNPKYNTFKEALKQRDGIAVIGIFLKIGHENGEFQIFLDALDKIKTKGKEAPFTKFDPSCLFPACRDYWTYQGSFTTPPCEECIVWLLLKEPMTVSSDQMAKLRSLLSSAENEPPVPLVSNWRPPQPINNRVVRASFK

28-30 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 25mM Tris, 150 mM NaCl, pH 8.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Brian P.Mahon, et al. Chapter 5 - Carbonic Anhydrase III. Carbonic Anhydrases as Biocatalysts, 2015, Pages 91-108.

  [2]. Han-Zhong Feng, et al. Carbonic Anhydrase III Is Expressed in Mouse Skeletal Muscles Independent of Fiber Type-Specific Myofilament Protein Isoforms and Plays a Role in Fatigue Resistance. Front Physiol. 2016 Dec 15;7:597.  [Content Brief]