Carbonic Anhydrase 4 蛋白, Human (HEK293, His)

Carbonic anhydrase 4 catalyzes the reversible hydration of carbon dioxide and plays a crucial role in maintaining intracellular and extracellular pH. Carbonic Anhydrase 4 Protein, Human (HEK293, His) is the recombinant human-derived Carbonic Anhydrase 4 protein, expressed by HEK293 , with C-His labeled tag. The total length of Carbonic Anhydrase 4 Protein, Human (HEK293, His) is 265 a.a., with molecular weight of ~30 kDa.

碳酸酐酶 4 (CA4) 通过催化二氧化碳可逆水合成碳酸氢盐和质子，在细胞生理学中发挥着至关重要的作用，这是维持细胞内和细胞外 pH 平衡所必需的过程。这种酶活性在各种研究中都有记录，强调了它在 pH 稳态中的重要性。CA4 还可能在刺激 SLC4A4 的钠/碳酸氢盐转运蛋白活性中发挥作用，从而有助于 pH 调节。特别值得注意的是它的重要功能是消除视网膜和视网膜上皮的酸超载，以及促进脉络膜内的脉络膜毛细血管中的酸释放。CA4 的多方面作用凸显了其对维持细胞 pH 稳态不可或缺的贡献，并强调了其在各种生理过程中的相关性。

Measured by its esterase activity and the specific activity is >2 pmoles/min/μg.

Human

HEK293

C-His

P22748 (A19-K283)

762  [NCBI]

MRMLLALLALSAARPSASAESHWCYEVQAESSNYPCLVPVKWGGNCQKDRQSPINIVTTKAKVDKKLGRFFFSGYDKKQTWTVQNNGHSVMMLLENKASISGGGLPAPYQAKQLHLHWSDLPYKGSEHSLDGEHFAMEMHIVHEKEKGTSRNVKEAQDPEDEIAVLAFLVEAGTQVNEGFQPLVEALSNIPKPEMSTTMAESSLLDLLPKEEKLRHYFRYLGSLTTPTCDEKVVWTVFREPIQLHREQILAFSQKLYYDKEQTVSMKDNVRPLQQLGQRTVIK

Approximately 30 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.