Carboxylesterase 5 蛋白, Mouse (HEK293, His)

Carboxylesterase 5 Protein, Mouse (HEK293, His) is a mouse Carboxylesterase 5 protein with a His-flag. Carboxylesterase 5 is encoded byCarboxylesterase 5 (CES5) and can catalyses hydrolytic and transesterification reactions^[1][2].

碳酸酐酶 11 属于碳酸酐酶 (CA) 家族，可能参与细胞增殖和细胞转化。CA 是锌金属酶，可催化二氧化碳的可逆水合，从而参与多种生物和物理过程^[1]。
羧酸酯酶 5 (CES5) 是至少五个哺乳动物 CES 基因家族之一，编码具有广泛底物特异性并催化水解和酯交换反应的酶^[2]。

Measured by its ability to hydrolyze 1mM 4-Nitrophenyl acetate that incubate at room temperature in kinetic mode for 5 minutes. The specific activity is 10573.649- 37513.038 pmol/min/μg.

Mouse

HEK293

C-6*His

Q8BK48 (Q27-R555)

234673  [NCBI]

QDSASPIRNTHTGQVRGSLVHVKDTDIAVHTFLGIPFAKPPVGPLRFAPPEAPEPWSGVRDGTSHPNMCLQNDNLMGSEDLKMMNLILPPISMSEDCLYLNIYVPAHAHEGSNLPVMVWIHGGALTVGMASMYDGSMLAATEDVVVVAIQYRLGVLGFFSTGDQHAKGNWGYLDQVAALRWVQQNIVHFGGNPDRVTIFGESAGGTSVSSHVVSPMSQGLFHGAIMESGVAVLPDLISSSSEMVHRIVANLSGCAAVNSETLMCCLRGKNEAEMLAINKVFKIIPGVVDGEFLPKHPQELMASKDFHPVPSIIGINNDEYGWILPTIMDPAQKIEEITRKTLPAVLKSTALKMMLPPECGDLLMEEYMGDTEDPETLQAQFREMKGDFMFVIPALQVAHFQRSHAPVYFYEFQHRPSFFKDFRPPYVKADHGDEIFLVFGYQFGNIKLPYTEEEEQLSRRIMKYWANFARHGNPNSEGLPYWPVMDHDEQYLQLDIQPSVGRALKARRLQFWTKTLPQKIQELKGSQERHHHHHHH

58-65 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against 20 mM Tris-HCl, 150 mM NaCl, pH8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Roger S Holmes, et al. Mammalian carboxylesterase 5: comparative biochemistry and genomics. 2008 Sep;3(3):195-204.  [Content Brief]