Carboxypeptidase B1/CPB1 蛋白, Human (HEK293, His)

Carboxypeptidase B1/CPB1 Protein, Human (HEK293, His) is an approximately 47.0 kDa carboxypeptidase B1 protein with a C-terminal His-flag.

羧肽酶 B1 由 CPB1 基因编码。推导的人 CPB1 氨基酸序列由信号肽（残基 1 至 15）、前区（残基 16 至 110）和成熟链（残基 111 至 417）组成^[1] .
羧肽酶 B (CPB) 是一种特异性水解肽 C 端碱性氨基酸的蛋白酶^[1]。因此，它在生物分析中发挥着重要作用。羧肽酶 B 在下调类风湿性关节炎炎症反应方面发挥着关键作用^[2]。

Measured by its ability to cleave a colorimetric peptide substrate, Hippuryl-Arg. The specific activity is 12990.93 pmol/min/µg.

Human

HEK293

C-6*His

P15086 (H16-Y417)

1360  [NCBI]

HHGGEHFEGEKVFRVNVEDENHINIIRELASTTQIDFWKPDSVTQIKPHSTVDFRVKAEDTVTVENVLKQNELQYKVLISNLRNVVEAQFDSRVRATGHSYEKYNKWETIEAWTQQVATENPALISRSVIGTTFEGRAIYLLKVGKAGQNKPAIFMDCGFHAREWISPAFCQWFVREAVRTYGREIQVTELLDKLDFYVLPVLNIDGYIYTWTKSRFWRKTRSTHTGSSCIGTDPNRNFDAGWCEIGASRNPCDETYCGPAAESEKETKALADFIRNKLSSIKAYLTIHSYSQMMIYPYSYAYKLGENNAELNALAKATVKELASLHGTKYTYGPGATTIYPAAGGSDDWAYDQGIRYSFTFELRDTGRYGFLLPESQIRATCEETFLAIKYVASYVLEHLYHHHHHH

Approximately 47.0 kDa

Greater than 95% as determined by reducing SDS-PAGE. 

溶液.

Supplied as a 0.2 μm filter solution of 20 mM MES, 150 mM NaCl, pH 5.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. K K Yamamoto, et al. Isolation of a cDNA encoding a human serum marker for acute pancreatitis. Identification of pancreas-specific protein as pancreatic procarboxypeptidase B. J Biol Chem. 1992 Feb 5;267(4):2575-81.  [Content Brief]

  [2]. J W Brodrick, et al. Human carboxypeptidase B. II. Purification of the enzyme from pancreatic tissue and comparison with the enzymes present in pancreatic secretion. Biochim Biophys Acta. 1976 Dec 8;452(2):468-81.  [Content Brief]