Carboxypeptidase M/CPM 蛋白, Human (HEK293, His)

Carboxypeptidase M Protein, Human (HEK293, His) is an approximately 55.0 kDa Carboxypeptidase M protein with a His-flag. Carboxypeptidase M is a membrane-bound arginine/lysine carboxypeptidase enzyme belongs to the carboxypeptidases family^[1].

羧肽酶 M 是一种膜结合的精氨酸/赖氨酸羧肽酶，可在中性 pH 最佳条件下裂解 C 末端碱性氨基酸^[1]。
羧肽酶 M 属于羧肽酶家族。羧肽酶 M 广泛分布于多种组织和细胞中，并在人体内特化细胞和组织表面以活性形式组成型表达^[2]。
在凝血/纤溶、炎症、食物消化以及激素原和神经肽加工等生理过程中发挥重要作用^[3]。

Measured by its ability to release L-arginine from Benzoyl-Ala-Arg, with detection of the arginine amino group by o-phthaldialdehyde. The specific activity is 187829.19 pmol/min/μg that incubate for 10 minutes at room temperature.

Human

HEK293

C-6*His

P14384 (L18-D421)

1368  [NCBI]

LDFNYHRQEGMEAFLKTVAQNYSSVTHLHSIGKSVKGRNLWVLVVGRFPKEHRIGIPEFKYVANMHGDETVGRELLLHLIDYLVTSDGKDPEITNLINSTRIHIMPSMNPDGFEAVKKPDCYYSIGRENYNQYDLNRNFPDAFEYNNVSRQPETVAVMKWLKTETFVLSANLHGGALVASYPFDNGVQATGALYSRSLTPDDDVFQYLAHTYASRNPNMKKGDECKNKMNFPNGVTNGYSWYPLQGGMQDYNYIWAQCFEITLELSCCKYPREEKLPSFWNNNKASLIEYIKQVHLGVKGQVFDQNGNPLPNVIVEVQDRKHICPYRTNKYGEYYLLLLPGSYIINVTVPGHDPHITKVIIPEKSQNFSALKKDILLPFQGQLDSIPVSNPSCPMIPLYRNLPDHHHHHHH

Approximately 55.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filter solution of 20 mM Tris-HCl, 150 mM NaCl, 1 mM ZnCl₂, pH7.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. A Nagae, et al. Carboxypeptidase M in brain and peripheral nerves. J Neurochem  [Content Brief]

  [2]. Kathleen Deiteren, et al. Carboxypeptidase M: Multiple alliances and unknown partners. Clin Chim Acta. 2009 Jan;399(1-2):24-39.  [Content Brief]

  [3]. David Reverter, Crystal structure of human carboxypeptidase M, a membrane-bound enzyme that regulates peptide hormone activity. J Mol Biol  [Content Brief]