Caspase-1/CASP1 Protein, Human (Active, T388A, His)

Caspase-1/CASP1 subunit p10 protein is a thiol protease complex involved in inflammation by catalyzing the cleavage of IL1B, IL18, and Gasdermin-D (GSDMD). It activates a pro-inflammatory response, releases mature cytokines IL1B and IL18, and initiates pyroptosis through cleavage of GSDMD. Caspase-1/CASP1 Protein, Human (Active, T388A, His) is the recombinant human-derived Caspase-1/CASP1, expressed by E. coli , with N-His labeled tag. ,

Caspase-1/CASP1 亚基 p10 蛋白是一种硫醇蛋白酶，通过催化与炎症相关的前体蛋白的蛋白水解裂解，复杂地参与各种炎症过程。这包括白细胞介素 1 β (IL1B) 和白细胞介素 18 (IL18)、关键炎症细胞因子以及细胞焦亡诱导剂 Gasdermin-D (GSDMD)，将它们转化为活性成熟肽。作为细胞免疫的关键启动子，Caspase-1 在炎症小体复合物形成时激活促炎反应，导致成熟细胞因子 IL1B 和 IL18 的释放，这些细胞因子在多种炎症途径中发挥着关键作用。Caspase-1 的活性延伸至通过裂解 GSDMD 来启动焦亡，这是一种程序性裂解细胞死亡途径。值得注意的是，与白细胞介素的裂解不同，GSDMD 的识别和裂解依赖于 CASP1 上的外切位点界面，这强调了其多功能且复杂的调节作用。此外，Caspase-1 响应细菌感染而激活 CASP7，促进质膜修复，并通过在 DNA 病毒感染期间激活炎症小体时裂解 CGAS 来控制抗病毒免疫。在凋亡细胞中，Caspase-1 会裂解 SPHK2，后者在细胞外仍具有酶活性。尽管 Caspase-1 参与多种细胞过程，但它对细胞凋亡不活跃。

Ac-YVAD-AMC is a synthetic tetrapeptide substrate that is cleaved between D and AMC by Caspase-1, releasing the fluorogenic AMC, which is detected by spectrofluorometry. 1U: The amount of enzyme required to consume 1μmol of substrate per minute at 25 °C. The CASP1 specific activity is 0.005146-0.006449 U/mg.

Human

E. coli

N-His

P29466-1 (N120-D297&A317-H404, T388A)

834

Approximately 32.0 KDa

Greater than 90 % as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 50 mM HEPES, pH 7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.