Cathepsin L1 蛋白, Mouse (HEK293, His)

Cathepsin L Protein, Mouse (HEK293, His) is a lysosomal acid cysteine protease, which is associated with tumor occurrence, development, and metastasis.

组织蛋白酶L（CTSL）是一种半胱氨酸蛋白酶，属于木瓜蛋白酶样家族（肽酶C1A），与肿瘤的发生、发展和转移有关。组织蛋白酶L与肿瘤的侵袭和转移、炎症状态、动脉粥样硬化、肾病、糖尿病、骨病、病毒感染等疾病有关。组织蛋白酶 L 的功能取决于其亚细胞定位：组织蛋白酶 L 参与细胞死亡和细胞质炎症，还在细胞核中调节细胞周期，并在细胞外环境中发挥降解作用。组织蛋白酶 L 在所有组织和细胞类型中表达，半胱氨酸组织蛋白酶的主要功能是水解病原体内吞作用产生的蛋白质抗原^[1][2]。

Measured by its ability to cleave the fluorogenic peptide substrate Z-LR-AMC. The specific activity is 26159.6 pmol/min/µg, as measured under the described conditions.

Mouse

HEK293

C-His

P06797 (T18-N334)

13039  [NCBI]

TPKFDQTFSAEWHQWKSTHRRLYGTNEEEWRRAIWEKNMRMIQLHNGEYSNGQHGFSMEMNAFGDMTNEEFRQVVNGYRHQKHKKGRLFQEPLMLKIPKSVDWREKGCVTPVKNQGQCGSCWAFSASGCLEGQMFLKTGKLISLSEQNLVDCSHAQGNQGCNGGLMDFAFQYIKENGGLDSEESYPYEAKDGSCKYRAEFAVANDTGFVDIPQQEKALMKAVATVGPISVAMDASHPSLQFYSSGIYYEPNCSSKNLDHGVLLVGYGYEGTDSNKNKYWLVKNSWGSEWGMEGYIKIAKDRDNHCGLATAASYPVVNHHHHHH

Approximately 36.02 kDa.

• 
  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, pH 7.5-8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Mei-Ling Han, et al. Cathepsin L upregulation-induced EMT phenotype is associated with the acquisition of cisplatin or paclitaxel resistance in A549 cells. Acta Pharmacol Sin. 2016 Dec;37(12):1606-1622.  [Content Brief]

  [2]. Caio P Gomes, et al. Cathepsin L in COVID-19: From Pharmacological Evidences to Genetics. Front Cell Infect Microbiol. 2020 Dec 8;10:589505.  [Content Brief]