Cathepsin L1 蛋白, Human (HEK293, C-His)

Cathepsin L1 protein is a thiol protease that plays a key role in lysosomal protein degradation, affecting cellular functions such as turnover, antigen processing, and bone remodeling. It dissolves cross-linked TG/thyroglobulin, releasing the thyroid hormone thyroxine (T4) in the thyroid follicular cavity. Cathepsin L1 Protein, Human (HEK293, C-His) is the recombinant human-derived Cathepsin L1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Cathepsin L1 Protein, Human (HEK293, C-His) is 316 a.a., with molecular weight of ~32.8 kDa.

组织蛋白酶 L1 蛋白是一种硫醇蛋白酶，在溶酶体内蛋白质的全面降解中发挥着关键作用，影响各种细胞功能，例如一般蛋白质周转、抗原加工和骨重塑。它的作用延伸到交联 TG/甲状腺球蛋白的溶解，通过甲状腺滤泡腔内有限的蛋白水解促进甲状腺激素甲状腺素 (T4) 的释放。此外，组织蛋白酶 L1 催化神经内分泌嗜铬细胞分泌小泡内的激素原脑啡肽加工成活性脑啡肽神经递质。在胸腺中，它通过生成 MHCII 结合肽配体来调节 CD4(+) T 细胞的阳性选择，并介导皮质胸腺上皮细胞中的不变链加工。组织蛋白酶 L1 在中性 pH 值下作为主要的弹性蛋白降解酶发挥作用，在抗原呈递细胞的细胞外空间中积聚，调节炎症期间细胞外基质的降解。此外，其分泌形式有助于 COL18A1 生成内皮抑素。除了其酶作用外，组织蛋白酶 L1 对于心脏形态和功能以及毛囊形态发生、循环和表皮分化也是不可或缺的。此外，它在最大化 TIMP1 刺激的类固醇生成方面发挥着至关重要的作用。在微生物感染的情况下，特别是在缺乏 TMPRSS2 表达的细胞中，组织蛋白酶 L1 通过冠状病毒刺突的蛋白水解作用，通过缓慢的酸激活途径，促进人类冠状病毒（例如 SARS-CoV 和 SARS-CoV-2）的进入 (S)溶酶体中的糖蛋白。这一活性强调了其在这些病毒感染宿主细胞中的重要性。

Measured by its ability to cleave the fluorogenic peptide substrate Z-LR-AMC. The specific activity is 121918.4296 pmol/min/µg, as measured under the described conditions.

Human

HEK293

C-6*His

P07711 (T18-V333)

1514

TLTFDHSLEAQWTKWKAMHNRLYGMNEEGWRRAVWEKNMKMIELHNQEYREGKHSFTMAMNAFGDMTSEEFRQVMNGFQNRKPRKGKVFQEPLFYEAPRSVDWREKGYVTPVKNQGQCGSCWAFSATGALEGQMFRKTGRLISLSEQNLVDCSGPQGNEGCNGGLMDYAFQYVQDNGGLDSEESYPYEATEESCKYNPKYSVANDTGFVDIPKQEKALMKAVATVGPISVAIDAGHESFLFYKEGIYFEPDCSSEDMDHGVLVVGYGFESTESDNNKYWLVKNSWGEEWGMGGYVKMAKDRRNHCGIASAASYPTVHHHHHH

Approximately 32.8 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM NaAC, 150 mM NaCl, pH 4.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.