Cathepsin L2 蛋白, Human (HEK293, His)

Cathepsin L2 Protein, Human (HEK293, His) is a functional cysteine proteinase, mainly expressed in the cornea, testis, thymus, and epidermis.

组织蛋白酶 L2（组织蛋白酶 V）是一种人半胱氨酸蛋白酶，与组织蛋白酶 L 具有很高的同一性（78%）。组织蛋白酶 L2 在人类肿瘤中的表达分析显示，在结直肠癌和乳腺癌中广泛表达，但在正常结肠癌或乳腺癌中不表达。乳腺或瘤周组织。组织蛋白酶 L2 也由结直肠癌细胞系和乳腺癌细胞系以及一些不同来源的肿瘤表达，包括卵巢癌和肾癌^[1][2]。

Measured by its ability to cleave the fluorogenic peptide substrate Z-LR-AMC and the specific activity is >1000 pmoles/min/μg, as measured under the described conditions.

Human

HEK293

C-6*His

O60911 (V18-V334)

1515  [NCBI]

VPKFDQNLDTKWYQWKATHRRLYGANEEGWRRAVWEKNMKMIELHNGEYSQGKHGFTMAMNAFGDMTNEEFRQMMGCFRNQKFRKGKVFREPLFLDLPKSVDWRKKGYVTPVKNQKQCGSCWAFSATGALEGQMFRKTGKLVSLSEQNLVDCSRPQGNQGCNGGFMARAFQYVKENGGLDSEESYPYVAVDEICKYRPENSVANDTGFTVVAPGKEKALMKAVATVGPISVAMDAGHSSFQFYKSGIYFEPDCSSKNLDHGVLVVGYGFEGANSNNSKYWLVKNSWGPEWGSNGYVKIAKDKNNHCGIATAASYPNVHHHHHH

Approximately 35-39 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized a 0.2 μm filtered solution of 20 mM MES, 150 mM NaCl, pH 5.5 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. I Santamaría, et al. Cathepsin L2, a novel human cysteine proteinase produced by breast and colorectal carcinomas. Cancer Res. 1998 Apr 15;58(8):1624-30.  [Content Brief]

  [2]. Sascha Hagemann, et al. The human cysteine protease cathepsin V can compensate for murine cathepsin L in mouse epidermis and hair follicles. Eur J Cell Biol. 2004 Dec;83(11-12):775-80.  [Content Brief]