CBR1 蛋白, Human (P.pastoris, His)

CBR1 is an NADPH-dependent reductase with broad substrate specificity and plays a key role in the reduction of various carbonyl compounds, including quinones, prostaglandins, menadione, and xenobiotics. Crucially, CBR1 converts the anthracyclines doxorubicin and daunorubicin into their cardiotoxic forms doxorubicin and daunorubicin. CBR1 Protein, Human (P.pastoris, His) is the recombinant human-derived CBR1 protein, expressed by P. pastoris , with N-6*His labeled tag. The total length of CBR1 Protein, Human (P.pastoris, His) is 276 a.a., with molecular weight of ~33.0 kDa.

CBR1 是一种 NADPH 依赖性还原酶，具有广泛的底物特异性，在多种羰基化合物（包括醌、前列腺素、甲萘醌和各种异生物质）的还原中发挥着关键作用。值得注意的是，CBR1 催化抗肿瘤蒽环类药物阿霉素和柔红霉素转化为其心脏毒性对应物阿霉素和柔红霉素。此外，它还展示了将前列腺素 E 转化为前列腺素 F2-α 的能力，突出了其在底物识别方面的多功能性。该酶与谷胱甘肽的相互作用通过与谷胱甘肽缀合底物的结合得到证明，进一步阐明了其对特定化合物的更高亲和力。此外，CBR1 通过促进 NADPH 依赖性的皮质醇/皮质酮转化为 20β-二氢皮质醇 (20b-DHF) 或 20β-皮质酮 (20b-DHB) 来参与糖皮质激素代谢，这两种物质都是脂肪中 NR3C1 和 NR3C2 的弱激动剂组织。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

P. pastoris

N-6*His

P16152 (S2-W277)

873  [NCBI]

SSGIHVALVTGGNKGIGLAIVRDLCRLFSGDVVLTARDVTRGQAAVQQLQAEGLSPRFHQLDIDDLQSIRALRDFLRKEYGGLDVLVNNAGIAFKVADPTPFHIQAEVTMKTNFFGTRDVCTELLPLIKPQGRVVNVSSIMSVRALKSCSPELQQKFRSETITEEELVGLMNKFVEDTKKGVHQKEGWPSSAYGVTKIGVTVLSRIHARKLSEQRKGDKILLNACCPGWVRTDMAGPKATKSPEEGAETPVYLALLPPDAEGPHGQFVSEKRVEQW

Approximately 33.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from 0.2 μm filtered solution in 20 mM Tris-HC1, 0.5 M NaCl, 3% Trehalose, pH 8.0.

<1.0 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.