CCDC47 蛋白, Human (HEK293, His)

The CCDC47 protein is an important component of the multichannel translocon (MPT) complex, which facilitates the insertion of multichannel membrane proteins into lipid bilayers. It blocks the lateral gate of the SEC61 complex, allowing insertion of subsequent transmembrane domains. CCDC47 Protein, Human (HEK293, His) is the recombinant human-derived CCDC47 protein, expressed by HEK293 , with C-His labeled tag.

CCDC47 蛋白是多通道易位子 (MPT) 复合物的重要组成部分，它有助于将多通道膜蛋白插入膜的脂质双层中。SEC61 复合物初始膜插入后，MPT 复合物接管并阻断 SEC61 复合物的侧门，促进后续跨膜区域的插入。在 MPT 复合物中，由 CCDC47 组成的 PAT 子复合物在隔离跨膜结构域中的高极性区域远离非极性膜环境中发挥作用，直到它们可以正确地埋入完全组装的蛋白质中。此外，CCDC47 阻断了 PAT 子复合体内 SEC61 复合体的侧门。CCDC47 还参与调节内质网 (ER) 中的钙离子稳态，并且对于通过 ER 相关降解 (ERAD) 途径进行适当的蛋白质降解至关重要。它的存在对于胚胎发生过程中维持内质网组织是必要的。CCDC47 与 WDR83OS/Asterix 一起形成 PAT 复合体，并且是较大 MPT 复合体的一部分，该复合体包括 GEL 复合体（RAB5IF/OPTI 和 TMCO1）、BOS 复合体（NCLN/Nicalin、NOMO 和 TMEM147）和 PAT 复合体。MPT 复合物与 SEC61 复合物相互作用，还与 VCP、HSPA5、DERL1、DERL2 和 SELENOS 相互作用。

Human

HEK293

C-6*His

Q96A33 (K21-S135)

57003  [NCBI]

KFDDFEDEEDIVEYDDNDFAEFEDVMEDSVTESPQRVIITEDDEDETTVELEGQDENQEGDFEDADTQEGDTESEPYDDEEFEGYEDKPDTSSSKNKDPITIVDVPAHLQNSWES

Approximately 19-23 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.2, 5% trehalose, 5% mannitol and 0.01% Tween80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.