CCL21C 蛋白, Mouse

CCL21C protein has dual functions of inhibiting hematopoiesis and inducing chemotaxis. In vitro, it selectively attracts thymocytes and activated T cells without affecting B cells, macrophages or neutrophils. CCL21C Protein, Mouse is the recombinant mouse-derived CCL21C protein, expressed by E. coli , with tag free. The total length of CCL21C Protein, Mouse is 110 a.a., with molecular weight of ~18 kDa.

CCL21C 蛋白通过抑制造血和诱导趋化作用发挥双重功能。在体外，它表现出特异性针对胸腺细胞和活化 T 细胞的趋化活性，对 B 细胞、巨噬细胞或中性粒细胞没有显着影响。此外，CCL21C 作为系膜细胞的有效化学引诱剂，并对初始 T 细胞表现出优先活性。CCL21C 与 CCR7 和 CXCR3 受体结合，暗示在淋巴细胞归巢至次级淋巴器官中发挥作用。此外，它与 PDPN 相互作用，导致 PDPN 重新定位到基底外侧膜。这些发现强调了 CCL21C 在免疫细胞行为和组织特异性反应中的多种调节功能。

Determined by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR7 .The ED₅₀ for this effect is 37.24 ng/mL, corresponding to a specific activity is 2.69×10^4 U/mg.

Mouse

E. coli

Tag Free

P86793 (S24-G133)

100042493  [NCBI]

SDGGGQDCCLKYSQKKIPYSIVRGYRKQEPSLGCPIPAILFLPRKHSKPELCANPEEGWVQNLMRRLDQPPAPGKQSPGCRKNRGTSKSGKKGKGSKGCKRTEQTQPSRG

Approximately 18 kDa band in SDS-PAGE under reducing conditions.

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in sterile distilled water.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.