MIP-1 alpha/CCL3 蛋白, Rat

The CCL3 protein is a single factor with inflammatory and chemotactic properties that attracts immune cells such as monocytes, neutrophils, eosinophils, basophils, and lymphocytes. It is critical in pulmonary TNF-α production, neutrophil recruitment, and lung injury and may serve as an autocrine mediator of TNF-α production by macrophages. MIP-1 alpha/CCL3 Protein, Rat is the recombinant rat-derived MIP-1 alpha/CCL3 protein, expressed by E. coli , with tag free.

CCL3 蛋白是一种具有炎症和趋化动力学特性的单因子，对多种免疫细胞（包括单核细胞、中性粒细胞、嗜酸性粒细胞、嗜碱性粒细胞和淋巴细胞）表现出趋化活性。它在肺 TNF-α 产生、中性粒细胞募集和随后的肺损伤中发挥着至关重要的作用，可能作为巨噬细胞产生 TNF-α 的自分泌介质。这反过来又上调了中性粒细胞流入所必需的血管粘附分子。此外，CCL3 蛋白表现出与肝素的结合亲和力。

1.Fully biologically active when compared to standard. The biological activity determined by a chemoattract bioassay using human blood monocytes is in a concentration range of 10-100 ng/mL.
2.Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR5. The ED₅₀ for this effect is 3.140 ng/mL, corresponding to a specific activity is 3.185×10⁵ U/mg.

• 
  Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR5.The ED₅₀ for this effect is 3.140 ng/mL, corresponding to a specific activity is 3.185×10⁵U/mg.

Rat

E. coli

Tag Free

P50229 (A24-A92)

25542  [NCBI]

APYGADTPTACCFSYGRQIPRKFIADYFETSSLCSQPGVIFLTKRNRQICADPKETWVQEYITELELNA

Approximately 7.9-10kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm solution of PBS, pH 7.4 or 50 mM Tris-HCL, 300 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in sterile distilled water.For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.