1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Chemokine & Receptors
  4. CC Chemokines
  5. MIP-1 beta/CCL4
  6. CCL4 蛋白, Rat

CCL4 蛋白, Rat

目录号: HY-P71899
COA 产品使用指南

CCL4 蛋白是一种单核因子,作为同型二聚体发挥作用,并表现出炎症和趋化动力学特性。CCL4 蛋白, Rat 是重组的 CCL4 蛋白,由 E. coli 表达,不带标签。CCL4 蛋白, Rat 全长 69 个氨基酸,分子量约为 ~11 kDa。

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规格 价格 是否有货 数量
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2 μg ¥350 In-stock
10 μg ¥950 In-stock
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100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

CCL4 protein, a monokine, acts as a homodimer and displays inflammatory and chemokinetic properties. CCL4 Protein, Rat is the recombinant rat-derived CCL4 protein, expressed by E. coli , with tag free. The total length of CCL4 Protein, Rat is 69 a.a., with molecular weight of ~11 kDa.

研究背景

CCL4 是一种单核因子,由于其作为同二聚体发挥作用,因此表现出炎症和趋化动力学特性。

生物活性

Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR5. The ED50 for this effect is 14.43 ng/mL, corresponding to a specific activity is 6.930×104 U/mg.

  • Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR5.The ED50 for this effect is 14.43 ng/mL, corresponding to a specific activity is 6.930×104 U/mg.
种属

Rat

表达系统

E. coli

标签

Tag Free

蛋白编号

P50230 (A24-N92)

基因 ID
蛋白结构
N-term
CCL4 (A24-N92)
Accession # P50230
C-term
同用名
Ccl4; Mip1b; Scya4C-C motif chemokine 4; Macrophage inflammatory protein 1-beta; MIP-1-beta; Small-inducible cytokine A4
氨基酸序列

APIGSDPPTSCCFSYTSRKIHRNFVMDYYETSSLCSQPAVVFLTKKGRQICADPSEPWVNEYVNDLELN

分子量

Approximately 11 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm solution of 50 mM Tris-HCL, 300 mM NaCl, pH 8.0 or PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in sterile distilled water.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CCL4 Protein, Rat
目录号:
HY-P71899
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