RANTES/CCL5 蛋白, Rat (His)

RANTES/CCL5 Protein, Rat (His) is a key pro-inflammatory chemokine in the CC chemokine family that interacts with CCR1, CCR3, CCR4, and CCR5 to mediate inflammatory immune responses, viral infections, and tumorigenesis. RANTES/CCL5 Protein, Rat (His) is a recombinant rat RANTES/CCL5 (S25-S92) protein expressed by  E. coli with a His tag at the C-terminu^[1].

CCL5，也称为RANTES（正常T细胞激活、表达和分泌的调节），属于趋化因子CC亚家族。CCL5基因位于人类17号染色体q11.2-q12区域，编码CCL5一种分子量为8 kDa的蛋白质。CCL5可由T细胞、单核细胞、NK细胞、上皮细胞、成纤维细胞表达，CCL5可与受体CCR1、CCR3、CCR4和CCR5结合，其中与CCR5的亲和力最高^[1]。CCL5 与 CCR5 结合导致磷脂酰肌醇 3 激酶 ( PI3K ) 磷酸化，磷酸化的 PI3K 进一步酸化丝氨酸 473 上的蛋白激酶 B，Akt/PKB 复合物磷酸化并灭活丝氨酸/苏氨酸蛋白激酶 GSK-3。同时，CCL5 与 CCR5 结合诱导 Bcl2 蛋白表达，从而促进细胞凋亡。CCL5 还可以充当 G 蛋白偶联受体 GPR75 的潜在激动剂，GPR75 与 GPR75 一起可能通过激活涉及 PI3、Akt 和 MAP 激酶的下游信号通路在神经元存活中发挥作用，并在胰腺的胰岛素分泌中发挥作用。通过激活 GPR75^[2] 胰岛细胞。除了充当趋化剂外，CCL5 也是 CD⁸⁺ T 细胞产生的主要 HIV 抑制因子。它参与炎症维持、移植、抗病毒免疫、肿瘤发展以及许多人类疾病和病症，例如病毒性肝炎或 COVID-19^[3]。

Rat

E. coli

N-6*His

P50231 (S25-S92)

81780  [NCBI]

HHHHHHSPYGSDTTPCCFAYLSLALPRAHVKEYFYTSSKCSNLAVVFVTRRNRQVCANPEKKWVQEYINYLEMS

Approximately 14 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against 20 mM PB, 500 mM NaCl, 2 mM EDTA, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. V Appay, et al. RANTES: a versatile and controversial chemokine. Trends Immunol. 2001 Feb;22(2):83-7.  [Content Brief]

  [2]. Rafael Elias Marques, et al. Targeting CCL5 in inflammation.  [Content Brief]

  [3]. Zhen Zeng, et al. CCL5/CCR5 axis in human diseases and related treatments. Genes Dis. 2022 Jan;9(1):12-27.

  [4]. Lili Chen, et al. Deletion of C-C Motif Chemokine Ligand 5 Worsens Invariant Natural Killer T-Cell-Mediated Hepatitis via Compensatory Up-regulation of CXCR2-Related Chemokine Activity. Cell Mol Gastroenterol Hepatol. 2019;7(3):623-639.  [Content Brief]

  [5]. F Cocchi, et al. Identification of RANTES, MIP-1 alpha, and MIP-1 beta as the major HIV-suppressive factors produced by CD8+ T cells. Science. 1995 Dec 15;270(5243):1811-5.  [Content Brief]

  [6]. Nahzli Dilek, et al. Control of transplant tolerance and intragraft regulatory T cell localization by myeloid-derived suppressor cells and CCL5. J Immunol. 2012 May 1;188(9):4209-16.  [Content Brief]

  [7]. Khalid Benamar, et al. Elevated level of the proinflammatory chemokine, RANTES/CCL5, in the periaqueductal grey causes hyperalgesia in rats. Eur J Pharmacol. 2008 Sep 11;592(1-3):93-5.  [Content Brief]