CCN2/CTGF 蛋白, Rat (HEK293)

The CCN2/CTGF protein is a major connective tissue mitochondrial attractant secreted by vascular endothelial cells and critical for chondrocyte proliferation and differentiation. Additionally, it mediates heparin- and divalent cation-dependent cell adhesion in various cell types, including fibroblasts, myofibroblasts, endothelial cells, and epithelial cells. CCN2/CTGF Protein, Rat (HEK293) is the recombinant rat-derived CCN2/CTGF protein, expressed by HEK293 , with tag free.

CCN2/CTGF 蛋白是一种主要的结缔组织线粒体吸引剂，由血管内皮细胞分泌。它对促进软骨细胞的增殖和分化起着至关重要的作用。此外，CCN2/CTGF 介导各种细胞类型（包括成纤维细胞、肌成纤维细胞、内皮细胞和上皮细胞）中肝素和二价阳离子依赖性细胞粘附。此外，它还能增强成纤维细胞生长因子诱导的 DNA 合成。CCN2/CTGF 作为单体存在并与 TSKU 相互作用。

Measured by its ability to mediate Balb/3T3 mouse embryonic fibroblast cell adhesion. The ED₅₀ for this effect is 1.921 μg/mL

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  Measured by its ability to mediate Balb/3T3 mouse embryonic fibroblast cell adhesion. The ED₅₀ for this effect is 1.921 μg/mL, corresponding to a specific activity is 521 units/mg.

Rat

HEK293

Tag Free

Q9R1E9 (Q25-A347)

64032  [NCBI]

QDCSAQCQCAAEAAPRCPAGVSLVLDGCGCCRVCAKQLGELCTERDPCDPHKGLFCDFGSPANRKIGVCTAKDGAPCVFGGSVYRSGESFQSSCKYQCTCLDGAVGCVPLCSMDVRLPSPDCPFPRRVKLPGKCCEEWVCDEPKDRTVVGPALAAYRLEDTFGPDPTMMRANCLVQTTEWSACSKTCGMGISTRVTNDNTFCRLEKQSRLCMVRPCEADLEENIKKGKKCIRTPKIAKPVKFELSGCTSVKTYRAKFCGVCTDGRCCTPHRTTTLPVEFKCPDGEIMKKNMMFIKTCACHYNCPGDNDIFESLYYRKMYGDMA

Approximately 35-43 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, pH 6.5, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 500 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.