NOV/CCN3 蛋白, Mouse (HEK293, His)

The NOV/CCN3 protein is an early player that coordinates diverse cellular processes such as proliferation, adhesion, migration, differentiation, and survival.By interacting with membrane receptors such as integrins or NOTCH1, it regulates hematopoietic stem cells and inhibits myogenic differentiation while affecting the expression of cell cycle regulators.NOV/CCN3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived NOV/CCN3 protein, expressed by HEK293 , with C-6*His labeled tag.

NOV/CCN3 蛋白作为早期参与者，在包括增殖、粘附、迁移、分化和生存在内的多种细胞过程中发挥着关键作用。NOV/CCN3 通过与整合素或膜受体（例如 NOTCH1）的结合相互作用而发挥作用，成为造血干细胞和祖细胞功能的重要调节剂。它通过激活 Notch 信号通路抑制肌源性分化，并独立于 TGFB1 信号传导抑制血管平滑肌细胞增殖，从而影响 CDKN2B 或 CDKN1A 等细胞周期调节因子的表达。作为整合素 ITGAV:ITGB3 和 ITGA5:ITGB1 的配体，NOV/CCN3 直接刺激促血管生成活性，诱导血管生成，并支持内皮细胞粘附、迁移和存活。此外，它通过与各种整合素对相互作用，在皮肤伤口愈合、皮肤成纤维细胞粘附和趋化性中发挥作用。此外，NOV/CCN3 有助于骨再生，负向调节关节软骨细胞表型，同时抑制软骨内骨化。它还影响胰腺 β 细胞功能，通过抑制 β 细胞增殖和胰岛素分泌发挥负调节作用。作为内皮促炎激活的负调节因子，它可减少单核细胞粘附，并通过抑制 NF-kappaB 信号通路发挥抗炎作用。NOV/CCN3 通过调节 IL1B 和 TNF 诱导的 MMP9、MMP2 和 CCL2 表达，以及通过与 ITGB1 结合抑制 MMP9 表达，进一步有助于控制动脉粥样硬化中的炎症过程并减轻炎症疼痛。它参与多种蛋白质相互作用，包括 FBLN1、NOTCH1、GJA1/CX43、ITGA5:ITGB1、ITGAV:ITGB3、ITGAV:ITGB5 和 ZDHHC22，可能导致 CCN3 棕榈酰化。这些相互作用的广度强调了 NOV/CCN3 在细胞过程和调控途径中复杂且多方面的作用。

Mouse

HEK293

C-6*His

Q64299 (S26-I354)

18133  [NCBI]

SLRCPSRCPPKCPSISPTCAPGVRSVLDGCSCCPVCARQRGESCSEMRPCDQSSGLYCDRSADPNNQTGICMVPEGDNCVFDGVIYRNGEKFEPNCQYFCTCRDGQIGCLPRCQLDVLLPGPDCPAPRKVAVPGECCEKWTCGSDEQGTQGTLGGLALPAYRPEATVGVEVSDSSINCIEQTTEWSACSKSCGMGVSTRVTNRNRQCEMVKQTRLCIVRPCEQEPEEVTDKKGKKCLRTKKSLKAIHLQFENCTSLYTYKPRFCGVCSDGRCCTPHNTKTIQVEFQCLPGEIIKKPVMVIGTCTCYSNCPQNNEAFLQDLELKTSRGEI

Approximately 50.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.