VCAM-1/CD106 蛋白, Human (HEK293, His)

CD106 Protein, Human (HEK293, His) is a cytokine-inducible cell surface protein capable of mediating adhesion.

CD106 (VCAM1) 是白细胞上存在的极晚期抗原 4 (VLA-4) 的配体。CD106 (VCAM1) 是神经干细胞生态位的一个组成部分，对于 MSC 介导的免疫抑制和造血祖细胞结合至关重要。CD106 促进白细胞与内皮细胞的牢固粘附。众所周知，CD106 介导的细胞间粘附对于 T 细胞激活和白细胞募集到炎症部位至关重要，因此它在引发有效的免疫反应方面发挥着重要作用。CD106 在绒毛膜绒毛 (CV)-MSC 上高表达，在 BM-MSC 上中等表达，在脐带 MSC 上表达较差，在脂肪 MSC 上不表达^[1]。

Human

HEK293

C-6*His

P19320 (F25-E698)

7412  [NCBI]

FKIETTPESRYLAQIGDSVSLTCSTTGCESPFFSWRTQIDSPLNGKVTNEGTTSTLTMNPVSFGNEHSYLCTATCESRKLEKGIQVEIYSFPKDPEIHLSGPLEAGKPITVKCSVADVYPFDRLEIDLLKGDHLMKSQEFLEDADRKSLETKSLEVTFTPVIEDIGKVLVCRAKLHIDEMDSVPTVRQAVKELQVYISPKNTVISVNPSTKLQEGGSVTMTCSSEGLPAPEIFWSKKLDNGNLQHLSGNATLTLIAMRMEDSGIYVCEGVNLIGKNRKEVELIVQEKPFTVEISPGPRIAAQIGDSVMLTCSVMGCESPSFSWRTQIDSPLSGKVRSEGTNSTLTLSPVSFENEHSYLCTVTCGHKKLEKGIQVELYSFPRDPEIEMSGGLVNGSSVTVSCKVPSVYPLDRLEIELLKGETILENIEFLEDTDMKSLENKSLEMTFIPTIEDTGKALVCQAKLHIDDMEFEPKQRQSTQTLYVNVAPRDTTVLVSPSSILEEGSSVNMTCLSQGFPAPKILWSRQLPNGELQPLSENATLTLISTKMEDSGVYLCEGINQAGRSRKEVELIIQVTPKDIKLTAFPSESVKEGDTVIISCTCGNVPETWIILKKKAETGDTVLKSIDGAYTIRKAQLKDAGVYECESKNKVGSQLRSLTLDVQGRENNKDYFSPEHHHHHH

85-120 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against 20 mM PB, 150 mM NaCl, 2 mM CaCl₂, 2 mM MgCl₂, 5% Threhalose, pH 7.2.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Shihong Lu, et al. CD106 is a novel mediator of bone marrow mesenchymal stem cells via NF-κB in the bone marrow failure of acquired aplastic anemia. Stem Cell Res Ther. 2017 Aug 1;8(1):178.  [Content Brief]