CD117/c-kit 蛋白, Human (HEK293, His)

CD117/c-kit is a tyrosine protein kinase receptor for KITLG/SCF that coordinates cell survival, proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration, and melanogenesis. Upon KITLG/SCF binding, CD117 activates the pathway and phosphorylates PIK3R1, PLCG1, SH2B2/APS, and CBL. CD117/c-kit Protein, Human (HEK293, His) is the recombinant human-derived CD117/c-kit protein, expressed by HEK293 , with C-6*His labeled tag.

CD117/c-kit 是一种酪氨酸蛋白激酶，作为细胞因子 KITLG/SCF 的细胞表面受体，在细胞存活、增殖、造血、干细胞维持、配子发生等细胞过程的协调中发挥着关键作用、肥大细胞发育、迁移和黑素生成。与 KITLG/SCF 结合后，KIT 激活多种信号通路，导致 PIK3R1、PLCG1、SH2B2/APS 和 CBL 磷酸化。AKT1 信号通路通过 PIK3R1（磷脂酰肌醇 3-激酶的调节亚基）的磷酸化被激活。此外，KIT 通过 GRB2 传输信号，激活 RAS、RAF1 和 MAP 激酶 MAPK1/ERK2 和/或 MAPK3/ERK1。此外，KIT 诱导 STAT 家族成员 STAT1、STAT3、STAT5A 和 STAT5B 的激活。PLCG1 激活导致二酰基甘油和肌醇 1,4,5-三磷酸的产生。蛋白磷酸酶调节 KIT 信号传导，受体经历快速内化和降解。激活的 KIT 促进涉及 PTPN6/SHP-1、PTPRU、STAT1、STAT3、STAT5A、STAT5B、PIK3R1、CBL、CRK（亚型 Crk-II）、LYN、MAPK1/ERK2 和/或 MAPK3/ERK1、PLCG1、SRC、和SHC1。

Human

HEK293

C-6*His

P10721-1 (Q26-T520)

3815  [NCBI]

QPSVSPGEPSPPSIHPGKSDLIVRVGDEIRLLCTDPGFVKWTFEILDETNENKQNEWITEKAEATNTGKYTCTNKHGLSNSIYVFVRDPAKLFLVDRSLYGKEDNDTLVRCPLTDPEVTNYSLKGCQGKPLPKDLRFIPDPKAGIMIKSVKRAYHRLCLHCSVDQEGKSVLSEKFILKVRPAFKAVPVVSVSKASYLLREGEEFTVTCTIKDVSSSVYSTWKRENSQTKLQEKYNSWHHGDFNYERQATLTISSARVNDSGVFMCYANNTFGSANVTTTLEVVDKGFINIFPMINTTVFVNDGENVDLIVEYEAFPKPEHQQWIYMNRTFTDKWEDYPKSENESNIRYVSELHLTRLKGTEGGTYTFLVSNSDVNAAIAFNVYVNTKPEILTYDRLVNGMLQCVAAGFPEPTIDWYFCPGTEQRCSASVLPVDVQTLNSSGPPFGKLVVQSSIDSSAFKHNGTVECKAYNDVGKTSAYFNFAFKGNNKEQIHPHT

Approximately 150 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.