CD14 蛋白, Cynomolgus (HEK293, His)

CD14 protein is a key coreceptor that cooperates with LBP to bind LPS and deliver it to the LY96/TLR4 complex to generate an immune response. CD14 activates NF-kappa-B through MyD88, TIRAP and TRAF6, triggering inflammation and cytokine release. CD14 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived CD14 protein, expressed by HEK293 , with C-His labeled tag. The total length of CD14 Protein, Cynomolgus (HEK293, His) is 344 a.a., with molecular weight of 45-56 kDa.

CD14 蛋白是细菌脂多糖 (LPS) 的关键共受体，与 LBP 合作结合单体 LPS 并将其递送至 LY96/TLR4 复合物，从而协调先天免疫反应。CD14 通过 MyD88、TIRAP 和 TRAF6 发挥作用，激活 NF-kappa-B，引发细胞因子释放和炎症。它还充当 TLR2:TLR6 和 TLR2:TLR1 异二聚体的共受体，分别响应二酰化和三酰化脂肽。此外，CD14 结合负电性 LDL，介导 LDL(-) 诱导的细胞因子释放。作为脂多糖 (LPS) 受体复合体（包括 CD14、LY96 和 TLR4）的成员，CD14 与 LPAR1 相互作用，突出了其在免疫和信号传导通路中的多功能作用。

Measured by its ability to enhance LPS-stimulated IL-8 secretion by THP-1 human acute monocytic leukemia cells. The ED₅₀ for this effect is 1.202 ng/mL, corresponding to a specific activity is 8.319×10⁵ U/mg.

Cynomolgus

HEK293

C-6*His

B3Y6B8 (T20-M344)

697482  [NCBI]

TTPEPCELDDEDFRCVCNFSEPHPDWSEAFQCVSAVEVEIRVGGLSLEPFLTRVDPDADPRQYADTIKALRVRRLTVGAAQVPAQLLVGALRVLAYSRLQELTLEDLEITGTMPPLPLEATGLALSSLRLHNVSWATGRSWLAELQQWLKPGLKVLSIAQAHSPAFSCEQVRAFPALTSLDLSDNPGLGERGLIAALCPHKFPALQNLALRNTGMETPTGVCAALAAAGVQPHSLDLSHNSLRATANPSAPRCMWSSALNSLNLSFAGLEQVPKGLPAKLRVLDLSCNRLNRRPRPDELPQVDNLALDGNPFLVPGTALPQEGSM

Approximately 45-56 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.