CD14 蛋白, Mouse (HEK293, His)

CD14 protein is a key protein in the innate immune response and is significantly expressed in monocytes/macrophages. It acts as a coreceptor that binds various microbial and fungal molecules, including lipopolysaccharide (LPS). CD14 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CD14 protein, expressed by HEK293 , with C-6*His labeled tag.

CD14 编码一种在先天免疫反应中至关重要的蛋白质，并在单核细胞/巨噬细胞中显着表达。作为辅助受体，它结合各种微生物和真菌分子，包括脂多糖（LPS）。LPS 结合蛋白 (LBP) 增强了该蛋白的 LPS 结合活性，促进与 TLR4-MD-2 共受体复合物的结合。CD14 以两种形式存在：可溶性形式和由糖基磷脂酰肌醇锚定物锚定在细胞表面的形式。它在乳腺和结肠等多种组织中广泛表达，突显了其在免疫相关过程中的关键作用。

Measured by its ability to enhance LPS-induced IL-6 secretion by CTLL-2 cells. The ED₅₀ this effect is 1.121 μg/mL, corresponding to a specific activity is 892.06 U/mg.

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  Measured by its ability to enhance LPS-induced IL-6 secretion by CTLL -2 cells. The ED₅₀ this effect is 1.121 μg/ml, corresponding to a specific activity is 892.06 U/mg.

Mouse

HEK293

C-6*His

P10810/NP_033971.1 (S16-P345)

12475  [NCBI]

MERVLGLLLLLLVHASPAPPEPCELDEESCSCNFSDPKPDWSSAFNCLGAADVELYGGGRSLEYLLKRVDTEADLGQFTDIIKSLSLKRLTVRAARIPSRILFGALRVLGISGLQELTLENLEVTGTAPPPLLEATGPDLNILNLRNVSWATRDAWLAELQQWLKPGLKVLSIAQAHSLNFSCEQVRVFPALSTLDLSDNPELGERGLISALCPLKFPTLQVLALRNAGMETPSGVCSALAAARVQLQGLDLSHNSLRDAAGAPSCDWPSQLNSLNLSFTGLKQVPKGLPAKLSVLDLSYNRLDRNPSPDELPQVGNLSLKGNPFLDSESHSEKFNSGVVTAGAPSSQAVALSGTLALLLGDRLFV

Approximately 45-50 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in PBS, pH 7.4. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.