CD150/SLAMF1 蛋白, Human (Biotinylated, HEK293, His-Avi)

CD150/SLAMF1 protein is an autoligand receptor in the SLAM family, which can regulate the activation and differentiation of immune cells and affect innate and adaptive immune responses. Its activity depends on the cytoplasmic adapters SH2D1A/SAP and/or SH2D1B/EAT-2. CD150/SLAMF1 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived CD150/SLAMF1 protein, expressed by HEK293 , with C-Avi, C-6*His labeled tag. The total length of CD150/SLAMF1 Protein, Human (Biotinylated, HEK293, His-Avi) is 217 a.a., with molecular weight of 45-65 kDa.

CD150/SLAMF1是一种属于信号淋巴细胞激活分子（SLAM）家族的自身配体受体，在调节各种免疫细胞的激活和分化中发挥着关键作用，从而有助于先天性和适应性免疫的复杂调节和协调回应。CD150 的活性受小细胞质接头蛋白（包括 SH2D1A/SAP 和/或 SH2D1B/EAT-2）是否存在的调节。与 B 细胞相比，CD150 在 T 淋巴细胞中诱导不同的信号转导事件，涉及两种信号转导模式 - 一种依赖于 SH2D1A（可能还有 SH2D1B），另一种依赖于蛋白酪氨酸磷酸酶 2C (PTPN11)。CD150 介导活化 T 细胞的不依赖于 IL-2 的增殖，诱导 IFN-γ 产生，并参与涉及 INPP5D、DOK1、DOK2、PRKCQ、BCL10 和 NFKB1 的下游信号通路。此外，它在滤泡辅助性T细胞的分化、过敏反应的调节以及巨噬细胞中针对革兰氏阴性菌的先天免疫反应的调节中发挥着至关重要的作用。此外，CD150 还可作为麻疹病毒（包括亚型 4）的受体。

Human

HEK293

C-Avi;C-6*His

Q13291-1 (A21-P237)

6504  [NCBI]

ASYGTGGRMMNCPKILRQLGSKVLLPLTYERINKSMNKSIHIVVTMAKSLENSVENKIVSLDPSEAGPPRYLGDRYKFYLENLTLGIRESRKEDEGWYLMTLEKNVSVQRFCLQLRLYEQVSTPEIKVLNKTQENGTCTLILGCTVEKGDHVAYSWSEKAGTHPLNPANSSHLLSLTLGPQHADNIYICTVSNPISNNSQTFSPWPGCRTDPSETKP

45-65 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.