CD163 蛋白, Human (HEK293, His-Avi)

The CD163 protein is an acute-phase regulatory receptor that clears hemoglobin/haptoglobin complexes through endocytosis by macrophages, protecting tissues from potential oxidative damage. In addition to iron uptake, CD163 also exhibits calcium- and pH-dependent binding preferences, with higher affinity for the hemoglobin/haptoglobin complex of the HP*1F phenotype. CD163 Protein, Human (HEK293, His-Avi) is the recombinant human-derived CD163 protein, expressed by HEK293 , with C-His, C-Avi labeled tag. The total length of CD163 Protein, Human (HEK293, His-Avi) is 1004 a.a., with molecular weight of 120-140 kDa.

CD163 是一种急性期调节受体，关键参与巨噬细胞对血红蛋白/触珠蛋白复合物的清除和内吞，从而保护组织免受游离血红蛋白引起的潜在氧化损伤。除了通过血红蛋白/结合珠蛋白的内吞作用在铁吸收和再循环中发挥作用之外，CD163 还以钙依赖性和 pH 依赖性方式与这些复合物结合。值得注意的是，与 HP*1S 表型的血红蛋白和二聚体触珠蛋白的复合物相比，它对 HP*1F 表型的血红蛋白和多聚体触珠蛋白的复合物表现出更高的亲和力。CD163 诱导一系列细胞内信号，涉及酪氨酸激酶依赖性钙动员、三磷酸肌醇产生以及 IL6 和 CSF1 的分泌。尤其是同工型 3，在细胞中表达时，显示出增强的配体内吞作用和表面表达。CD163 的脱落产生具有潜在抗炎特性的可溶形式 (sCD163)，可作为监测炎症条件下巨噬细胞活化的有价值的诊断参数。

Human

HEK293

C-His;C-Avi

Q86VB7-1 (S42-S1045)

9332  [NCBI]

SSLGGTDKELRLVDGENKCSGRVEVKVQEEWGTVCNNGWSMEAVSVICNQLGCPTAIKAPGWANSSAGSGRIWMDHVSCRGNESALWDCKHDGWGKHSNCTHQQDAGVTCSDGSNLEMRLTRGGNMCSGRIEIKFQGRWGTVCDDNFNIDHASVICRQLECGSAVSFSGSSNFGEGSGPIWFDDLICNGNESALWNCKHQGWGKHNCDHAEDAGVICSKGADLSLRLVDGVTECSGRLEVRFQGEWGTICDDGWDSYDAAVACKQLGCPTAVTAIGRVNASKGFGHIWLDSVSCQGHEPAIWQCKHHEWGKHYCNHNEDAGVTCSDGSDLELRLRGGGSRCAGTVEVEIQRLLGKVCDRGWGLKEADVVCRQLGCGSALKTSYQVYSKIQATNTWLFLSSCNGNETSLWDCKNWQWGGLTCDHYEEAKITCSAHREPRLVGGDIPCSGRVEVKHGDTWGSICDSDFSLEAASVLCRELQCGTVVSILGGAHFGEGNGQIWAEEFQCEGHESHLSLCPVAPRPEGTCSHSRDVGVVCSRYTEIRLVNGKTPCEGRVELKTLGAWGSLCNSHWDIEDAHVLCQQLKCGVALSTPGGARFGKGNGQIWRHMFHCTGTEQHMGDCPVTALGASLCPSEQVASVICSGNQSQTLSSCNSSSLGPTRPTIPEESAVACIESGQLRLVNGGGRCAGRVEIYHEGSWGTICDDSWDLSDAHVVCRQLGCGEAINATGSAHFGEGTGPIWLDEMKCNGKESRIWQCHSHGWGQQNCRHKEDAGVICSEFMSLRLTSEASREACAGRLEVFYNGAWGTVGKSSMSETTVGVVCRQLGCADKGKINPASLDKAMSIPMWVDNVQCPKGPDTLWQCPSSPWEKRLASPSEETWITCDNKIRLQEGPTSCSGRVEIWHGGSWGTVCDDSWDLDDAQVVCQQLGCGPALKAFKEAEFGQGTGPIWLNEVKCKGNESSLWDCPARRWGHSECGHKEDAAVNCTDISVQKTPQKATTGRS

120-140 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.