CD19 蛋白, Mouse (HEK293, His)

The CD19 protein acts as a coreceptor for B cell antigen receptors, lowering the signaling threshold and initiating B cell responses to antigens. It activates a cascade leading to PI3K activation and Ca(2+) mobilization. CD19 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CD19 protein, expressed by HEK293 , with C-His labeled tag. The total length of CD19 Protein, Mouse (HEK293, His) is 271 a.a., with molecular weight of ~51 kDa.

CD19 蛋白作为 B 淋巴细胞上 B 细胞抗原受体复合物 (BCR) 的辅助受体，有效降低下游信号通路的阈值并启动 B 细胞对抗原的反应。它激活信号级联，导致磷脂酰肌醇 3-激酶激活和细胞内 Ca(2+) 储备的动员。虽然 CD19 对于骨髓中的早期 B 细胞分化不是必需的，但它对于 B-1 细胞的正常分化至关重要，并且在响应抗原挑战的 B 细胞分化和增殖中发挥着至关重要的作用。此外，CD19对于维持血清免疫球蛋白的正常水平以及在抗原暴露后产生高亲和力抗体是必需的。CD19 与 CR2/CD21 相互作用，并与成熟 B 细胞膜上的 CD81、CR2/CD21、CD81 和 IFITM1/CD225 形成复合物。它还与 CD81 直接相互作用，当特定酪氨酸残基磷酸化时，CD81 对于细胞表面 CD19 受体的运输和区室化至关重要。此外，当 Tyr-402 和 LYN 磷酸化时，CD19 与 PLCG2 相互作用；当酪氨酸磷酸化时，CD19 与磷脂酰肌醇 3-激酶 (PI3K) 的调节性 p85 亚基相互作用。

Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD19 is present at 2 μg/mL, can bind Recombinant biotinylated Human CD81. The ED₅₀ for this effect is 8.41 μg/mL.

Mouse

HEK293

C-His

P25918 (G17-G287)

12478  [NCBI]

GGRPQKSLLVEVEEGGNVVLPCLPDSSPVSSEKLAWYRGNQSTPFLELSPGSPGLGLHVGSLGILLVIVNVSDHMGGFYLCQKRPPFKDIWQPAWTVNVEDSGEMFRWNASDVRDLDCDLRNRSSGSHRSTSGSQLYVWAKDHPKVWGTKPVCAPRGSSLNQSLINQDLTVAPGSTLWLSCGVPPVPVAKGSISWTHVHPRRPNVSLLSLSLGGEHPVREMWVWGSLLLLPQATALDEGTYYCLRGNLTIERHVKVIARSAVWLWLLRTGG

Approximately 45-70 kDa due to the glycosylation.

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 6.5-7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.