CD229/SLAMF3 蛋白, Human (HEK293, His)

The CD229/SLAMF3 protein is an autoligand receptor in the SLAM family that regulates immune cell activation and differentiation through cell interactions. Essential for innate and adaptive immune responses, its activity is controlled by SH2D1A/SAP and/or SH2D1B/EAT-2. CD229/SLAMF3 Protein, Human (HEK293, His) is the recombinant human-derived CD229/SLAMF3 protein, expressed by HEK293 , with C-6*His labeled tag.

CD229/SLAMF3 蛋白是一种自身配体受体，属于信号淋巴细胞激活分子 (SLAM) 家族。它通过同型或异型细胞间相互作用充当免疫细胞激活和分化的调节剂。该受体在调节先天性和适应性免疫反应中发挥着至关重要的作用。小细胞质接头蛋白 SH2D1A/SAP 和/或 SH2D1B/EAT-2 的存在或不存在控制其活性。CD229/SLAMF3 通过同源相互作用参与 T 淋巴细胞和辅助细胞之间的粘附反应。它促进 T 细胞分化为辅助 T 细胞 Th17 表型，导致 IL-17 分泌增加，共刺激活性需要 SH2D1A。此外，它还促进 RORC 招募至 IL-17 启动子。CD229/SLAMF3 还可能充当免疫反应的负调节因子，有助于维持外周细胞耐受性。它可以抑制自身抗体反应和CD4(+) T细胞分泌IFN-γ。此外，它还参与调节胸腺先天性 CD8(+) T 细胞的大小和恒定自然杀伤 T (iNKT) 细胞的发育。CD229/SLAMF3 与 SH2D1A、SH2D1B、INPP5D 和 PTPN11 相互作用，与 PTPN11 的相互作用被 SH2D1A 阻断。

Human

HEK293

C-6*His

Q9HBG7-1 (K48-K454)

4063  [NCBI]

KDSAPTVVSGILGGSVTLPLNISVDTEIENVIWIGPKNALAFARPKENVTIMVKSYLGRLDITKWSYSLCISNLTLNDAGSYKAQINQRNFEVTTEEEFTLFVYEQLQEPQVTMKSVKVSENFSCNITLMCSVKGAEKSVLYSWTPREPHASESNGGSILTVSRTPCDPDLPYICTAQNPVSQRSSLPVHVGQFCTDPGASRGGTTGETVVGVLGEPVTLPLALPACRDTEKVVWLFNTSIISKEREEAATADPLIKSRDPYKNRVWVSSQDCSLKISQLKIEDAGPYHAYVCSEASSVTSMTHVTLLIYRRLRKPKITWSLRHSEDGICRISLTCSVEDGGNTVMYTWTPLQKEAVVSQGESHLNVSWRSSENHPNLTCTASNPVSRSSHQFLSENICSGPERNTK

Approximately 75 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.