CD23/Fc epsilon RII 蛋白, Mouse (HEK293, His)

CD23/Fc epsilon RII Protein, a low-affinity receptor for IgE and CR2/CD21, regulates IgE production and B cell differentiation. On B cells, it initiates IgE-dependent antigen uptake and presentation to T cells. On macrophages, IgE binding induces intracellular killing of parasites through the L-Arginine-nitric oxide pathway. CD23/Fc epsilon RII homotrimer interacts with IGHE to regulate IgE homeostasis and with CR2/CD21 for binding. CD23/Fc epsilon RII Protein, Mouse (HEK293, His) is the recombinant mouse-derived CD23/Fc epsilon RII protein, expressed by HEK293 , with N-His labeled tag. The total length of CD23/Fc epsilon RII Protein, Mouse (HEK293, His) is 282 a.a., with molecular weight of 33-43 kDa.

CD23/Fc epsilon RII 蛋白作为免疫球蛋白 E (IgE) 和 CR2/CD21 的低亲和力受体，在 IgE 产生和 B 细胞分化的调节中发挥关键作用。在 B 细胞上，它启动 IgE 依赖性抗原摄取并呈递给 T 细胞，有助于免疫反应调节。在巨噬细胞中，CD23 在与 IgE 结合并与抗原交联后，通过激活 L-精氨酸-一氧化氮途径触发细胞内杀死寄生虫。CD23 作为同源三聚体存在，与 IGHE 相互作用，通过其 C 型凝集素结构域调节 IgE 稳态。此外，CD23 通过其 C 末端与 CR2/CD21 相互作用，通过 Sushi 结构域 1 和 2 形成连接。这些相互作用强调了 CD23 在免疫过程中的多方面参与，影响 B 细胞功能和巨噬细胞介导的反应。

Immobilized recombinant mouse CD23 at 5 μg/ml (100 μl/well) can bind Biotinylated Recombinant mouse IgE Protein. The ED₅₀ for this effect is 0.1294 μg/mL.

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  Immobilized recombinant mouse CD23 at 5 μg/ml (100 μl/well) can bind Biotinylated Recombinant mouse IgE Protein. The ED₅₀ for this effect is 0.1294 μg/mL.

Mouse

HEK293

N-His

P20693-1 (E50-P331)

14128  [NCBI]

33-43 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.