CD299 蛋白, Human (HEK293, hFc)

The CD299 protein is an important C-type lectin that plays a role in cell adhesion and pathogen recognition. It can identify pathogens such as Mycobacterium tuberculosis, Ebola virus, hepatitis C, HIV-1, influenza A, West Nile virus and SARS-CoV. CD299 Protein, Human (HEK293, Fc) is the recombinant human-derived CD299 protein, expressed by HEK293 , with N-hFc labeled tag.

CD299 是一种编码 C 型凝集素的基因，在细胞粘附和病原体识别中发挥着至关重要的作用。该受体具有广泛的特异性，可识别对公共卫生具有重大影响的多种病原体，包括结核分枝杆菌、埃博拉病毒、丙型肝炎、HIV-1、甲型流感、西尼罗河病毒和 SARS-CoV 急性呼吸综合征冠状病毒。该蛋白质结构包含四个不同的结构域：C 端碳水化合物识别结构域、可变长度的柔性串联重复颈结构域、跨膜区和参与内化的 N 端细胞质结构域。CD299 与其邻近基因 CD209（也称为 DC-SIGN）具有密切的序列和功能相似性，尽管它们在病毒识别和表达模式方面有所不同。CD299 在肝脏、淋巴结和胎盘的内皮细胞中表现出高表达。值得注意的是，串联重复颈结构域的多态性与对 SARS 感染的抵抗力有关。由于在肝脏 (RPKM 15.4) 和淋巴结 (RPKM 9.8) 等组织中观察到了偏向表达，CD299 在免疫反应和病原体防御中发挥着关键作用。

Measured by its binding ability in a functional ELISA. When Recombinant ICAM-3 Protein（HY-P72921）is immobilized at 2 µg/mL (100 µL/well), can bind Recombinant Human CD299 Protein. The ED₅₀ for this effect is 1.304 μg/mL.

Human

HEK293

N-hFc

Q9H2X3-1/NP_055072.3 (S78-E399)

10332

SLSQEQSEQDAIYQNLTQLKAAVGELSEKSKLQEIYQELTQLKAAVGELPEKSKLQEIYQELTRLKAAVGELPEKSKLQEIYQELTRLKAAVGELPEKSKLQEIYQELTRLKAAVGELPEKSKLQEIYQELTELKAAVGELPEKSKLQEIYQELTQLKAAVGELPDQSKQQQIYQELTDLKTAFERLCRHCPKDWTFFQGNCYFMSNSQRNWHDSVTACQEVRAQLVVIKTAEEQNFLQLQTSRSNRFSWMGLSDLNQEGTWQWVDGSPLSPSFQRYWNSGEPNNSGNEDCAEFSGSGWNDNRCDVDNYWICKKPAACFRDE

Approximately 65-77 kDa

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.