CD300LF 蛋白, Human (His)

The CD300LF protein acts as a multifunctional immunomodulator, acting as an inhibitory receptor on myeloid cells and mast cells. It plays a vital role in immune homeostasis by actively regulating the phagocytosis of apoptotic cells by binding to phosphatidylserine. CD300LF Protein, Human (His) is the recombinant human-derived CD300LF protein, expressed by E. coli , with N-6*His labeled tag.

CD300LF 蛋白在免疫系统中充当多方面的调节剂，充当骨髓细胞和肥大细胞的抑制性受体。它通过识别并结合凋亡细胞表面的磷脂酰丝氨酸 (PS)，积极调节凋亡细胞的吞噬作用或胞吞作用，从而在免疫稳态中发挥关键作用。CD300LF 还通过与神经酰胺和鞘磷脂结合，充当 Fc ε 受体依赖性肥大细胞激活和过敏反应的负调节因子。此外，它还可以作为白细胞介素 4 (IL-4) 的辅助受体，增强 IL-4 和 IL-13 诱导的信号传导（通过相似性）。CD300LF 通过激活磷酸酶 PTPN6/SHP-1 和 PTPN11/SHP-2 负向调节 MYD88 和 TRIF 介导的 Toll 样受体 (TLR) 信号传导。此外，它还能抑制破骨细胞形成并诱导巨噬细胞死亡（通过相似性）。该蛋白以酪氨酸磷酸化依赖性方式与 PTPN6/SHP-1 相互作用，并与 IL4R 相关（通过相似性）。

Human

E. coli

N-6*His

Q8TDQ1-1 (T20-S156)

146722  [NCBI]

TQITGPTTVNGLERGSLTVQCVYRSGWETYLKWWCRGAIWRDCKILVKTSGSEQEVKRDRVSIKDNQKNRTFTVTMEDLMKTDADTYWCGIEKTGNDLGVTVQVTIDPAPVTQEETSSSPTLTGHHLDNRHKLLKLS

Approximately 21 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.