1. 重组蛋白
  2. CD Antigens Receptor Proteins
  3. Macrophage CD Proteins Stem Cell CD Proteins Platelet CD Proteins Erythrocyte CD Proteins Endothelial cell CD Proteins Cell Adhesion Molecules (CAMs)
  4. CD31/PECAM-1 Immunoglobulin-like Cell Adhesion Molecules
  5. CD31/PECAM-1 蛋白, Human

CD31/PECAM-1 蛋白是一种关键的细胞粘附分子,对于大多数炎症条件下的白细胞跨内皮迁移 (TEM) 至关重要。PECAM-1 调节缓激肽受体 BDKRB2 激活,并调节内皮细胞中缓激肽和高渗休克诱导的 ERK1/2 激活。通过 PECAM-1 的信号传导导致中性粒细胞、单核细胞和白细胞的激活。CD31/PECAM-1 蛋白, Human 是重组的 CD31/PECAM-1 蛋白,由 HEK293 表达,不带标签。

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MCE 顾客使用本产品发表的科研文献

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

CD31/PECAM-1 Protein, a pivotal cell adhesion molecule, is essential for leukocyte transendothelial migration (TEM) in most inflammatory conditions. PECAM1 modulates bradykinin receptor BDKRB2 activation and regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells. Signaling through PECAM-1 leads to the activation of neutrophils, monocytes and leukocytes. CD31/PECAM-1 Protein, Human is the recombinant human-derived CD31/PECAM-1 protein, expressed by HEK293 , with tag free.

研究背景

CD31/PECAM-1 是一种关键的细胞粘附分子,对于大多数炎症条件下的白细胞跨内皮迁移 (TEM) 至关重要。Tyr-679 在 TEM 中的关键作用强调了它需要 PECAM-1 往返于侧缘回收区室 (LBRC) 的有效运输,这对于靶向迁移白细胞周围的 LBRC 膜至关重要。跨亲同性相互作用可能通过细胞连接促进内皮细胞与细胞的粘附。PECAM-1 的同种连接具有双重目的,通过传递分离信号来防止巨噬细胞介导的邻近活白细胞的吞噬作用,同时通过将凋亡白细胞束缚到吞噬细胞来促进巨噬细胞介导的对凋亡白细胞的吞噬作用。值得注意的是,PECAM-1 调节缓激肽受体 BDKRB2 激活,并调节内皮细胞中缓激肽和高渗休克诱导的 ERK1/2 激活。它与各种伙伴 (包括 BDKRB2、GNAQ、PTPN11、FER 和 CD177) 的相互作用进一步阐明了控制其多种细胞功能的复杂分子机制,而其反式同二聚化对于有效的细胞间相互作用至关重要[1][2] [3][4][5]

生物活性

Measured by its ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 5×104 cells/well are added to rhCD31 coated plates (3.3 μg/mL,100 μL/well) in the presence of 20 ng/mL PMA, approximately 80.4% will adhere after 30 minutes at 37°C.

种属

Human

表达系统

HEK293

标签

Tag Free

蛋白编号

EAW94208.1 (Q28-K601)

基因 ID
蛋白结构
N-term
CD31 (Q28-K601)
Accession # EAW94208.1
C-term
同用名
Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; PECA1; CD31
氨基酸序列

QENSFTINSVDMKSLPDWTVQNGKNLTLQCFADVSTTSHVKPQHQMLFYKDDVLFYNISSMKSTESYFIPEVRIYDSGTYKCTVIVNNKEKTTAEYQVLVEGVPSPRVTLDKKEAIQGGIVRVNCSVPEEKAPIHFTIEKLELNEKMVKLKREKNSRDQNFVILEFPVEEQDRVLSFRCQARIISGIHMQTSESTKSELVTVTESFSTPKFHISPTGMIMEGAQLHIKCTIQVTHLAQEFPEIIIQKDKAIVAHNRHGNKAVYSVMAMVEHSGNYTCKVESSRISKVSSIVVNITELFSKPELESSFTHLDQGERLNLSCSIPGAPPANFTIQKEDTIVSQTQDFTKIASKSDSGTYICTAGIDKVVKKSNTVQIVVCEMLSQPRISYDAQFEVIKGQTIEVRCESISGTLPISYQLLKTSKVLENSTKNSNDPAVFKDNPTEDVEYQCVADNCHSHAKMLSEVLRVKVIAPVDEVQISILSSKVVESGEDIVLQCAVNEGSGPITYKFYREKEGKPFYQMTSNATQAFWTKQKANKEQEGEYYCTAFNRANHASSVPRSKILTVRVILAPWKK

分子量

Approximately 115 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 10 mM NaCl, pH 7.5.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CD31/PECAM-1 Protein, Human
目录号:
HY-P72892
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