2. 重组蛋白
  3. Enzymes & Regulators
  4. Hydrolases (EC 3)
  5. CD39L1/ENTPD2 蛋白, Human (sf9, His)

CD39L1/ENTPD2 蛋白, Human (sf9, His)

目录号: HY-P76903
产品使用指南

CD39L1/ENTPD2 蛋白在神经系统中表达，可有效水解 ATP 和各种核苷酸，同时对 ADP 的水解有限。其底物特异性层次突出了其在嘌呤能信号传导和选择性核苷酸水解中的调节作用，有助于精确控制神经传递。CD39L1/ENTPD2 蛋白, Human (sf9, His) 是重组的 CD39L1/ENTPD2 蛋白，由 Sf9 insect cells 表达，带有 N-His 标签。CD39L1/ENTPD2 蛋白, Human (sf9, His) 全长 432 个氨基酸，分子量约为 ~59 kDa。

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描述

CD39L1/ENTPD2 protein, expressed in the nervous system, efficiently hydrolyzes ATP and various nucleotides, while showing limited hydrolysis of ADP. Its substrate specificity hierarchy highlights its regulatory role in purinergic signaling and selective nucleotide hydrolysis, contributing to precise control of neurotransmission. CD39L1/ENTPD2 Protein, Human (sf9, His) is the recombinant human-derived CD39L1/ENTPD2 protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of CD39L1/ENTPD2 Protein, Human (sf9, His) is 432 a.a., with molecular weight of ~59 kDa.
研究背景

CD39L1/ENTPD2蛋白主要在神经系统中表达，通过有效水解ATP和各种核苷酸，在嘌呤能神经传递的调节中发挥关键作用。值得注意的是，CD39L1/ENTPD2 仅表现出 ADP 的边缘水解，表明其底物特异性将其与更广泛的核苷酸水解区分开来。不同底物的酶活性顺序揭示了优先等级，其中 ATP 被最有效地水解，其次是 GTP、CTP、ITP 和 UTP，而 ADP 和 UDP 显示出相当低的水解效率。这种微妙的底物特异性强调了 CD39L1/ENTPD2 在调节神经系统内嘌呤能信号通路中的复杂调节作用，强调了它通过选择性核苷酸水解对神经传递的精细控制的贡献。
种属

Human
表达系统

Sf9 insect cells
标签

N-His
蛋白编号

Q9Y5L3 (T29-D460)
基因 ID

954  [NCBI]
蛋白结构
N-term
His
ENTPD2 (T29-D460)
Accession # Q9Y5L3
C-term
同用名
Ectonucleoside triphosphate diphosphohydrolase 2; Entpd2; Cd39l1
分子量

Approximately 59 kDa
纯度

Greater than 85% as determined by reducing SDS-PAGE
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

CD39L1/ENTPD2 Protein, Human (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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