CD3E-CD3G Heterodimer 蛋白, Cynomolgus (HEK293, Fc)

CD3E/CD3 epsilon 1-27 peptide, crucial in the TCR-CD3 complex, transmits signals during T-cell activation. As APCs activate the TCR, CD3E, with ITAMs, undergoes LCK/FYN-mediated phosphorylation, initiating downstream signaling. Beyond signaling, CD3E contributes to T-cell development, forming CD3D/CD3E and CD3G/CD3E heterodimers. It regulates TCR-CD3 internalization through endocytosis sequences and interacts with CD6, NCK1, and NUMB, essential for internalization and degradation. The TCR-CD3 complex, including CD3D/CD3E and CD3G/CD3E heterodimers and CD3Z homodimers, demonstrates versatility in TCR assembly, allowing substitution of TCRalpha and TCRbeta with TCRgamma and TCRdelta. CD3E-CD3G Heterodimer Protein, Cynomolgus (HEK293, Fc) is a recombinant protein dimer complex containing cynomolgus-derived CD3E-CD3G Heterodimer protein, expressed by HEK293, with C-hFc labeled tag. CD3E-CD3G Heterodimer Protein, Cynomolgus (HEK293, Fc), has molecular weight of 36.9 kDa(CD3E) & 36.5 kDa(CD3G), respectively.

CD3E/CD3 epsilon 1-27 肽是 T 淋巴细胞表面 TCR-CD3 复合物的组成部分，在适应性免疫反应中发挥着至关重要的作用。被抗原呈递细胞 (APC) 激活后，T 细胞受体 (TCR) 通过 CD3 链跨细胞膜传递信号，包括 CD3D、CD3E、CD3G 和 CD3Z，每个链都包含基于免疫受体酪氨酸的激活基序 (ITAM)在它们的细胞质结构域中。Src 家族蛋白酪氨酸激酶 LCK 和 FYN 对这些 ITAM 进行磷酸化，一旦 TCR 参与，就会激活下游信号通路。除了在信号转导中的作用之外，CD3E 对于正确的 T 细胞发育也是不可或缺的，它通过形成异二聚体 CD3D/CD3E 和 CD3G/CD3E 来启动 TCR-CD3 复合物的组装。此外，CD3E 通过其胞质区域的内吞序列参与 TCR-CD3 复合物的内化和细胞表面下调。TCR-CD3复合物包含CD3D/CD3E和CD3G/CD3E异二聚体，优先与TCRα和TCRβ缔合，形成三聚体。然后，该六聚体与 CD3Z 同二聚体相互作用，形成 TCR-CD3 复合物。或者，TCRalpha和TCRbeta可以被TCRgamma和TCRdelta替代。CD3E/CD3 epsilon 1-27 肽还与 CD6、NCK1 和 NUMB 相互作用，其中 NUMB 相互作用对于 TCR-CD3 内化和随后的降解至关重要。

Measured by its binding ability in a functional ELISA. When Cynomolgus CD3E&CD3Gl is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Anti-CD3 Antibody binds with an EC₅₀ of 54.9 ng/mL.

Cynomolgus

HEK293

C-hFc

Q95LI5 (Q22-D117)&Q95LI7 (Q23-T113)

102133065  [NCBI]&102134381  [NCBI]

36.9 kDa(CD3E) & 36.5 kDa(CD3G)

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

<0.02 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.