CD4 蛋白, Ferret (HEK293, His)

CD4 Protein, an integral membrane glycoprotein, plays a pivotal role in immune responses. In T-cells, it serves as a coreceptor for MHC class II molecules, interacting with the T-cell receptor and initiating intracellular signaling pathways. In macrophages and NK cells, CD4 contributes to differentiation, cytokine expression, and cell migration independently of TCR/LCK. Additionally, CD4 is crucial in T-helper cell development, monocyte differentiation, and acts as the primary receptor for HIV-1, down-regulated by HIV-1 Vpu, and Human Herpesvirus 7/HHV-7. CD4 Protein, Ferret (HEK293, His) is the recombinant CD4 protein, expressed by HEK293 , with C-His labeled tag. The total length of CD4 Protein, Ferret (HEK293, His) is 376 a.a., with molecular weight of ~52 kDa.

CD4 蛋白是一种完整的膜糖蛋白，在免疫反应中发挥着至关重要的作用，承担着应对外部和内部挑战的多种功能。在 T 细胞中，其主要功能是作为 MHC II 类分子：肽复合物的辅助受体，其中 II 类肽源自细胞外蛋白，而 I 类肽源自胞质蛋白。CD4 同时与 T 细胞受体 (TCR) 和抗原呈递细胞 (APC) 呈递的 MHC II 类相互作用，导致 Src 激酶 LCK 募集到 TCR-CD3 复合物附近。随后，LCK 通过磷酸化不同的底物启动各种细胞内信号传导途径，最终导致淋巴因子的产生、增强的运动性、粘附性和 T 辅助细胞的激活。在其他细胞类型 (例如巨噬细胞或 NK 细胞) 中，CD4 通过 TCR/LCK 独立途径促进分化/激活、细胞因子表达和细胞迁移。此外，它在胸腺中 T 辅助细胞的发育中起着关键作用，并触发单核细胞分化为功能性成熟巨噬细胞。值得注意的是，CD4 作为人类免疫缺陷病毒 1 (HIV-1) 的主要受体，其下调由 HIV-1 Vpu 促进，并且它还作为人类疱疹病毒 7/HHV-7 的受体^[1][2][3]。

Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. When 5×10⁴ cells/well are added to CD4-coated plates (1.25μg/mL and 100μL/well), approximately 53.82% will adhere specifically after 30 minutes at 37°C.

Others

HEK293

C-His

I6LI22 (R26-L401)

101684487  [NCBI]

REVVLGKVGDTAELPCNGSVGQNIVFNWMQSTVKILGRQINFWITGASKLKNRVESKKNLWDQGSFPLIIKNLEAADSGVYFCEVDGKKQAVELLVFNLTAKWNTGSSSGGSNIRLLQGQQLTLTVEAPSGSSPSVQWKGPGNKSKGSGHRLSLSGLDVQESGTWTCTISQNQKTVVFNINILVLGFQKVSNTVYSREGEQVKFSFPLNFEIEKLSGELRWRTEGAPSSLLWSSFTLENKKLFVKEVHHPRLQLKETLPLSFILPQASSQYAGSGTLTLSLAKGTLQQEVNLVVMRVTKFTNNLTCEVMGPTSPELTLSLNLEERAAKVSKQQKLVSVVEPEDGTWQCLLSDKDKVLLASKVEVLSPVFTKAWPKL

Approximately 52 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.